R5403

Monoclonal Anti-Rhodopsin antibody produced in mouse

clone 1D4, purified immunoglobulin, buffered aqueous solution

• 동의어(들): Anti-CSNBAD1, Anti-OPN2, Anti-RP4
• MDL number: MFCD01865244
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 1D4, monoclonal
• 형태: buffered aqueous solution
• 종 반응성: human, rat, bovine
• 기술: immunocytochemistry: 1:1,000 using human retinal samples; western blot: 1:1,000 using Sf9 cells expressing the bovine gene
• 동형: IgG1
• UniProt 수납 번호: P08100
• 배송 상태: wet ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... RHO(6010) ; rat ... Rho(24717)

설명

일반 설명

Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin′s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin.

특이성

By Western blot, mouse monoclonal clone 1D4 anti-Rhodopsin antibody detects an ~40 kDa protein representing rhodopsin from Sf 9 cells expressing the bovine gene. Immunocytochemical staining of rhodopsin in human retinal samples results in staining of both rod and cone outer segments.

The epitope for this antibody has been localized to the C-terminal nine amino acids of bovine rhodopsin known as the 1D4 epitope, and detects rhodopsin from human and bovine retinal samples.

면역원

bovine rhodopsin.

애플리케이션

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Mouse monoclonal clone 1D4 anti-Rhodopsin antibody has been successfully used in Western blot, immunocytochemistry, and immunoprecipitation. It is used as a probe to determine the presence and roles of rhodopsin in the conversion of light into electrochemical signals.

물리적 형태

Solution in phosphate buffered saline containing 1 mg/mL BSA and 0.05% sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable