R5028

Monoclonal Anti-hnRNP-C1/C2 antibody produced in mouse

clone 4F4, purified immunoglobulin, buffered aqueous solution

• 동의어(들): Anti-Heterogeneous Nuclear Ribonucleoprotein-C1/C2
• MDL number: MFCD06798703
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 4F4, monoclonal
• 양식: buffered aqueous solution
• 분자량: antigen 41 kDa; antigen 43 kDa
• 종 반응성: monkey, human, chicken, hamster
• 기술: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.1-0.2 μg/mL using HeLa cell nuclear extract
• 동형: IgG1
• UniProt 수납 번호: P07910
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... HNRNPC(3183)

설명

일반 설명

Monoclonal Anti-hnRNP-C1/C2 (mouse IgG1isotype) is derived from the 4F4 hybridoma produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with RNPs eluted from oligo (dT) cellulose column. Heterogeneous nuclear ribonucleoproteins C1/C2 (HnRNP C1/C2) are synthesized from C2 protein by alternative splicing and have an additional13-amino-acids. It is localized in nucleus and has leucine zipper-like RNA-binding motif. The hnRNP-C proteins have a single ribonucleoprotein (RNP) motif RNA-binding domain (RBD) of 80 to 100 amino acid long.

면역원

RNPs eluted from oligo (dT) cellulose column.

애플리케이션

Monoclonal Anti-hnRNP-C1/C2 antibody produced in mouse has been used in:

• antibody microarray
• western blotting
• enzyme linked immunosorbent assay (ELISA)`
• immunoprecipitation
• immunocytochemistry

생화학적/생리학적 작용

Heterogeneous nuclear ribonucleoproteins C1/C2(HnRNP C1/C2) is involved in protein translation, mRNA biogenesis and transport. The hnRNP-C proteins preferentially bind to uridine-rich RNA sequences. Oligomerization of the protein through leucine rich regions in its C-terminal end is important for RNA binding. Mutation of the hnRNPC gene is implicated in embryonic lethal phenotype.

물리적 형태

Solution in phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable