R1656

RAF1 (EE) (306-end), active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• 동의어(들): CMD1NN, NS5, RAF proto-oncogene serine/threonine-protein kinase, cRaf
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: fermentation/recombinant
• Quality Level: 200
• 재조합: expressed in baculovirus infected Sf9 cells
• 제품 라인: PRECISIO^® Kinase
• 분석: ≥70% (SDS-PAGE)
• 양식: buffered aqueous glycerol solution
• 특이 활성도: 5100-6900 nmol/min·mg
• 분자량: ~63 kDa
• 제조업체/상표: Precision
• 기술: cell based assay: suitable
• solubility: water: soluble
• 단백질 ID 수납 번호: NM 002880
• UniProt 수납 번호: P04049
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... RAF1(5894)

설명

일반 설명

Research area: Cell Signaling

Rapidly Accelerated Fibrosarcoma (RAF) belongs to receptor tyrosine kinase (RTK) family. RAF exists as three isoforms A, B and C-RAF or RAF-1. RAF1 links Ras family members with mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) signaling cascade. The RAF-1 gene is mapped to the human chromosome at 3p25.2.

생화학적/생리학적 작용

Rapidly Accelerated Fibrosarcoma 1(RAF1), a member of MAP kinase kinase kinase (MAP3K) family plays a vital role in cell signaling by directly interacting with GTPases and functions downstream in their signaling pathway. RaF1 is activated by Ras in the presence of growth-promoting signals, leading to the activation of mitogen-activated protein kinase kinase (MEK1/MEK2) and extracellular signal-regulated protein kinases 1 and 2 (ERK1/ERK2) downstream. The activated ERK proteins control gene expression related to cell division, apoptosis, cell differentiation, and cell migration. The gene is mutated or upregulated in prostate and bladder cancer. Also, mutations are associated with Noonan syndrome, which leads to various physical abnormalities like craniofacial dysmorphisms, ocular, and musculoskeletal irregularities, neurocognitive impairment, and cardiac malformations.

물리적 형태

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

법적 정보

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)