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Key Documents

PURD10005

Sigma-Aldrich

Pur-A-Lyzer Midi Dialysis Kit

Midi 1000, capacity 50-800 μL, MWCO 1 kDa

동의어(들):

Dialyzers, High through put dialysis tubing, puralyzer

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About This Item

UNSPSC 코드:
41123100
NACRES:
NB.22

material

regenerated cellulose membrane

설명

includes floating rack and handbook

포장

pkg of 5 Maxi dialysis tubes

용량

50-800 μL

튜브 길이 × 직경

5.2 cm × 1.8 cm

공극 크기

1 kDa MWCO

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일반 설명

Pur-A-Lyzer dialysis tube kits are easy-to-use devices in a screw-cap tube format and are disposable. They contain a dual dialysis membrane window for buffer exchange. Samples are added and removed using a standard laboratory pipette and come in 4 different versions to accommodate a range of sample volumes.

  • High sample recovery >97%
  • Protease-, RNase-, and DNase-free
  • Convenient sample loading- no syringes necessary
  • Versatile- dialyze proteins, RNA, and DNA
The Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield.

The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.

The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.

애플리케이션

  • Dialysis or buffer exchange of volumes between 10 mL and 20 mL
  • Preparation of protein samples for MALDI-MS
  • Sample concentration
  • Large-scale protein dialysis such as antibodies and recombinant protein purification
  • Peptide dialysis for molecules as small as 10 amino acids
  • Virus-particle purification
  • Removal of contaminating micro molecules
  • Purification of tissue culture extracts
  • Removal of salts, surfactants, solvents and detergents
  • Complex formation studies (protein-protein, protein-DNA and protein-RNA)
  • pH and buffer adjustment of sample solution, protein extract or cell extract
  • High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad

For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10-250 μL
Midi: 50-800 μL
Maxi: 0.1-3 mL
Mega: 3-20 mL

법적 정보

Pur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLC

시험 성적서(COA)

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문서 라이브러리 방문

Bhagyashree D Rao et al.
Chemistry and physics of lipids, 226, 104849-104849 (2019-12-15)
pH (low) insertion peptide (pHLIP) is a polypeptide from the third transmembrane helix of bacteriorhodopsin. The pH-dependent membrane insertion of pHLIP has been conveniently exploited for translocation of cargo molecules and as a novel imaging agent in cancer biology due

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