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Merck
모든 사진(1)

Key Documents

P9318

Sigma-Aldrich

Monoclonal Anti-Plectin antibody produced in mouse

clone 7A8, ascites fluid

동의어(들):

Anti-EBS1, Anti-EBS5A, Anti-EBS5B, Anti-EBS5C, Anti-EBS5D, Anti-EBSMD, Anti-EBSND, Anti-EBSO, Anti-EBSOG, Anti-EBSPA, Anti-HD1, Anti-LGMD2Q, Anti-LGMDR17, Anti-PCN, Anti-PLEC1, Anti-PLEC1b, Anti-PLTN

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

mouse

결합

unconjugated

항체 형태

ascites fluid

항체 생산 유형

primary antibodies

클론

7A8, monoclonal

분자량

antigen 300 kDa

포함

15 mM sodium azide

종 반응성

marsupial (Ptoorous tridactylis PtK2 cell line), rat

기술

dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:200 using unfixed frozen sections of rat heart
microarray: suitable
western blot: suitable

동형

IgG1

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

일반 설명

Monoclonal Anti-Plectin (mouse IgG1 isotype) is derived from the 7A8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Plectin is an abundant, high molecular weight, cytomatrix protein (300 kDa) found in a wide variety of tissue and cell types. Plectin is found in stratified and non-stratified epithelia, fibroblasts, endothelial cells and astrocytes, as well as in striated, smooth and cardiac muscle, but not in neurons.

특이성

The antibody recognizes an epitope located in the middle section of the rod domain of the plectin molecule, approximately halfway between the globular end domains. The kinase C phosphorylation site was found on the same terminal segment as this antibody epitope. The antibody specifically labels the 300 kDa band of plectin, however, lower M.W. band(s) may also be detected. The antibody stains filamentous structures on immunohistochemical staining of frozen tissue sections. The antibody inhibits the interaction of plectin with vimentin and lamin B. An additional application for this antibody is immunoelectronmicroscropy.

면역원

plectin from rat glioma C6 cells

애플리케이션

Monoclonal Anti-Plectin antibody produced in mouse has been used in:
  • ELISA
  • immunoblot
  • dot blot
  • immunocytochemistry
  • immunoelectron microscopy

생화학적/생리학적 작용

Plectin plays a role in the cross-linking of intermediate filaments, the interlinking of intermediate filaments with microtubules and microfilaments and the anchoring of intermediate filaments to the plasma membrane and the nuclear membrane. It is a part of the signal transduction mechanism involving kinases A and C, because in vitro as well as in vivo phosphorylation of the protein by those kinases differentially affected its binding affinities to vimentin and lamin B.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

Plectin-1 as a novel biomarker for pancreatic cancer
Bausch D, et al.
Clinical Cancer Research, 17(2), 302-309 (2011)
Plectin isoforms as organizers of intermediate filament cytoarchitecture
Wiche G and Winter L
Bioarchitecture, 1(1), 14-20 (2011)
Peter Fuchs et al.
Cellular and molecular neurobiology, 25(7), 1141-1150 (2006-01-05)
The widely expressed cytolinker protein plectin shows extensive isoform diversity both at the N-terminus and in the central part of the molecule. Judged on mRNA data, plectin variants lacking the central rod domain are expressed at a approximately 20-fold lower
Daisuke Tsuruta et al.
The Journal of biological chemistry, 278(40), 38707-38714 (2003-07-18)
Within each hemidesmosome, alpha6beta4 integrin plays a crucial role in hemidesmosome assembly by binding to laminin-5 in the basement membrane zone of epithelial tissue. Recent analyses have implicated "specificity-determining loops" (SDLs) in the I-like domain of beta integrin in regulating
Marc Aristaeus de Asis et al.
Spermatogenesis, 3(3), e25733-e25733 (2014-01-02)
Tubulobulbar complexes (TBCs) are actin-related endocytic structures that internalize intercellular junctions in the seminiferous epithelium. The structures consist of elongate tubular projections of the attached plasma membranes of two adjacent cells that project into Sertoli cells. This double membrane core

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