P5542
Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus
lyophilized powder, ≥1,000 units/mg protein (Lowry)
동의어(들):
1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC
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모든 사진(3)
About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.32
추천 제품
형태
lyophilized powder
Quality Level
특이 활성도
≥1,000 units/mg protein (Lowry)
외래 활성
Phopholipase C (lecithinase) ≤1 units/mg protein
Sphingomyelinase ≤40 units/mg protein
저장 온도
−20°C
유전자 정보
Bacillus cereus E33L ... BCZK3513(3026815)
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI.
애플리케이션
Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane. It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells.
생화학적/생리학적 작용
Used for the release of GPI anchored proteins from the membrane.
단위 정의
One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).
물리적 형태
Contains phosphate buffer salts, EDTA and stabilizer
분석 메모
Acetylcholinesterase is measured according to Ellman, et al.
억제제
제품 번호
설명
가격
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Helen Neuert et al.
Development (Cambridge, England), 147(2) (2019-12-22)
The development of tissues and organs requires close interaction of cells. To achieve this, cells express adhesion proteins such as the neural cell adhesion molecule (NCAM) or its Drosophila ortholog Fasciclin 2 (Fas2). Both are members of the Ig-domain superfamily
Imaging of mobile long-lived nanoplatforms in the live cell plasma membrane.
Brameshuber M
The Journal of Biological Chemistry, 285(53), 41765-41771 (2010)
Danielle K Bailey et al.
PloS one, 16(7), e0254794-e0254794 (2021-07-27)
Iron and other transition metals, such as copper and manganese, are essential for supporting brain function, yet over-accumulation is cytotoxic. This over-accumulation of metals, particularly iron, is common to several neurological disorders; these include Alzheimer's disease, Parkinson's disease, Friedrich's ataxia
Crystal structure of the phosphatidylinositol-specific phospholipase C from Bacillus cereus in complex with myo-inositol.
Heinz DW
The Embo Journal, 14(16), 3855-3863 (1995)
Role of placental alkaline phosphatase in the internalization of trypomatigotes of Trypanosoma cruzi into HEp2 cells.
Sartori MJ
Tropical Medicine & International Health, 8(9), 832-839 (2003)
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