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Merck
모든 사진(1)

주요 문서

P3775

Sigma-Aldrich

Anti-Protein A antibody produced in rabbit

fractionated antiserum, lyophilized powder

동의어(들):

Anti-Protein A

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

rabbit

Quality Level

결합

unconjugated

항체 형태

fractionated antiserum

항체 생산 유형

primary antibodies

클론

polyclonal

양식

lyophilized powder

포장

vial of 2 mL lyophilized antiserum

기술

indirect ELISA: 1:100,000

저장 온도

2-8°C

타겟 번역 후 변형

unmodified

일반 설명

Protein A is a 42 kDa bacterial protein. It is isolated from the cell wall of Staphylococcus aureus.
The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. To ensure specificity the fractionated antiserum is adsorbed using solid phase techniques, if necessary. Rabbit Anti-Protein A is lyophilized from 0.01 M phosphate buffered saline, pH 7.2, to which no preservatives have been added. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion versus anti-rabbit IgG results in a single arc of precipitation and versus anti-rabbit whole serum results in multiple arcs of precipitation.

면역원

Protein A from Staphylococcus aureus

애플리케이션

Anti-Protein A antibody produced in rabbit has been used in immunoblotting.
Protein A may be used as in immunoblotting with a minimum dilution of 1:50,000.

생화학적/생리학적 작용

Protein A has an ability to bind Fc region of immunoglobulins from several species. It is used as a “universal tracer” and it facilitate the segregation of bound and free ligand. Protein A also acts as affinity matrices for antibody (IgG) purification.

물리적 형태

Lyophilized from 0.01M phosphate buffered saline, pH 7.2

재구성

Reconstitute with 2 ml deionized water.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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문서 라이브러리 방문

Human IgG subclasses
Antibody Fc, 159-177 (2014)
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PloS one, 7(10), e46864-e46864 (2012-10-12)
Most mitochondrial mRNAs in trypanosomatid parasites require uridine insertion/deletion RNA editing, a process mediated by guide RNA (gRNA) and catalyzed by multi-protein complexes called editosomes. The six oligonucleotide/oligosaccharide binding (OB)-fold proteins (KREPA1-A6), are a part of the common core of
Jitendra Thakur et al.
Eukaryotic cell, 10(10), 1295-1305 (2011-05-17)
A fungus-specific outer kinetochore complex, the Dam1 complex, is essential in Saccharomyces cerevisiae, nonessential in fission yeast, and absent from metazoans. The reason for the reductive evolution of the functionality of this complex remains unknown. Both Candida albicans and Schizosaccharomyces
IMMUNOASSAY CONFIGURATIONS
Immunoassay (1996)
Cell cycle-dependent phosphorylation and ubiquitination of a G protein alpha subunit
Torres MP, et al.
The Journal of Biological Chemistry, 286(23), 20208-20216 (2011)

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