추천 제품
제품명
Phosphate Buffered Saline with 0.05% TWEEN® 20, pH 7.4,
양식
powder
Quality Level
solubility
water: soluble, clear, colorless (1 pouch dissolved in 1L)
응용 분야
cell analysis
diagnostic assay manufacturing
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일반 설명
Phosphate buffered saline with TWEEN® 20, or PBS-T, is primarily used in immunoassays as a washing and blocking buffer. The phosphate buffer maintains a stable pH 7.4, suitable for many biological reactions and assays. The salt or saline solution in PBS-T provides the necessary ionic strength for optimal protein solubility and stability. The addition of 0.05% TWEEN 20, a non-ionic detergent, helps reduce background signals by blocking non-specific binding sites on proteins. PBS-T buffer enhances the washing efficiency by removing unwanted substances and limits non-specific antibody binding sites, while preserving the integrity of the target proteins.
애플리케이션
Phosphate buffered saline has been used in:
- western blotting
- enzyme-linked immunosorbent assay (ELISA)
- telomere-FISH (Tel-FISH) and telomere/immunostaining-FISH (Teli-Fish)
- cell isolation
포장
Foil pouches
재구성
Contents of one pouch dissolved in 1 L dH2O or ultrapure water to yield 0.01 M phosphate buffered saline, 0.138M NaCl, 0.0027M KCl, and 0.05% Tween 20, pH7.4.
기타 정보
For additional information on our range of Biochemicals, please complete this form.
법적 정보
TWEEN is a registered trademark of Croda International PLC
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
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