P3369

Protamine sulfate

solid, 90.0-110.0% dry basis,≥92% (chromatography), suitable for transduction

• 동의어(들): Salmine sulfate from salmon sperm, Protamine sulfate
• MDL number: MFCD00132091
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• Product Name: Protamine sulfate, meets USP testing specifications
• Agency: meets USP testing specifications
• Quality Level: 200
• 분석: ≥92% (chromatography); 90.0-110.0% dry basis
• 양식: solid
• 분자량: M_r 5000-10000
• 기술: transduction: suitable
• 배송 상태: ambient
• 저장 온도: 2-8°C

설명

일반 설명

Protamine sulfate is a cationic polypeptide having a molecular weight of 5 kDa, isolated from salmon sperm.

애플리케이션

Protamine sulfate has been used in the transduction of

• lentiviruses into human embryonic 293T cells
• shRNA into mesenchymal stem cells/marrow stromal cells (MSC)
• lenti Sca1-OCT4 plasmid into human cord blood hematopoietic progenitor CD34⁺ cells

생화학적/생리학적 작용

Alkaline protamine binds to negatively charged heparin. Use of protamine during cardiovascular surgery sometime triggers anaphylactic reactions.

Protamine facilitates direct transfection of microbial cells, and of human cells using cationic liposomes. Protamine effectively neutralizes the anticoagulation effects of heparin.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)