P2545

Protein A–Agarose

saline suspension

• 동의어(들): Protein A resin
• MDL number: MFCD00165563
• UNSPSC 코드: 41106500
• NACRES: NA.56

속성

• 생물학적 소스: Staphylococcus aureus
• Quality Level: 200
• 양식: saline suspension
• 라벨링 범위: ~2 mg per mL
• 기질 활성: cyanogen bromide
• 기질 부착: amino
• 기질 스페이서: 1 atom
• 용량: ≥15 mg/mL binding capacity (human IgG)
• 저장 온도: 2-8°C

설명

일반 설명

Protein A is a 42-kDa protein from Staphylococcus aureus and has five immunoglobulin binding domains.

애플리케이션

Protein A−Agarose has been used in the immunoprecipitation

• of protein phosphatase and tensin homolog (PTEN) protein from S63 and NIH3T3 fibroblast cells
• of prolyl hydroxylase domain 2 N27 cells
• of Wnt3a and sonic hedgehog (Shh) in mouse fibroblast L-cells and human embryonic kidney (HEK) 293 cells

Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.

생화학적/생리학적 작용

Protein A binds to Fcγ (the constant region) and Fab (antigen binding fragment) of IgG. Protein A agarose resin is commercially used for affinity based purifications.

물리적 형태

Suspension in 0.5 M NaCl containing preservative

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)