추천 제품
결합
unconjugated
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
pT-154, monoclonal
농도
~2 mg/mL
기술
direct ELISA: suitable
immunohistochemistry: suitable
microarray: suitable
western blot: 2-4 μg/mL using total extract of A431 cells stimulated by EGF
동형
IgG2b
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
관련 카테고리
일반 설명
Anti-Phosphotyrosine antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the pT-154 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a tyrosine phosphorylated peptide. Members of protein-tyrosine kinases (PTKs) family consists of extra-cellular domain that is responsible for ligand binding, a trans-membrane domain, modular domain and an intracellular domain.
면역원
tyrosine phosphorylated peptide.
애플리케이션
Anti-Phosphotyrosine antibody, Mouse monoclonal has been used in:
- enzyme linked immunosorbent assay (ELISA)
- western blot
- immunohistochemistry
생화학적/생리학적 작용
Protein phosphorylation is a post-translational modification that regulates signal transduction. Phosphorylation of tyrosine residues in proteins has been associated with oncogenesis, cell growth and survival . Monoclonal Anti-Phosphotyrosine antibody is specific for rat and human proteins/peptides that contain phosphotyrosine residues. As determined by competitive ELISA, the product does not bind to non-phosphorylated tyrosine, phosphorylated serine or threonine, ATP or AMP.
Protein-tyrosine kinases (PTKs) are enzymes that catalyze the transfer of Γ-phosphate of ATP to tyrosine residues of protein substrates. The PTKs are responsible for many biological processes like cell cycle, proliferation, oncogenesis and development. They are tightly regulated by other kinases and by autophosphorylation activity. Monoclonal antibodies specific for phosphotyrosine are essential tools for the study of tyrosine phosphorylation in post-translational modification in many biological processes.
물리적 형태
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Hanuman P Yadav et al.
Theriogenology, 96, 164-171 (2017-05-24)
The beneficial effects of cholesterol loaded cyclodextrin (CLC) addition were evaluated in cryopreserved bull semen. Forty ejaculates were collected from Hariana bulls (n = 4), pooled and divided into 4 aliquots. All the aliquots were initially diluted in to egg yolk tris
Protein tyrosine kinase structure and function
Hubbard SR and Till JH
Annual Review of Biochemistry, 69(1), 373-398 (2000)
Tyrosine kinase-role and significance in cancer
Paul MK and Mukhopadhyay AK
International Journal of Medical Sciences, 1(2), 101-101 (2004)
Debrup Sengupta et al.
The EMBO journal, 38(16), e99266-e99266 (2019-07-05)
During MHC-I-restricted antigen processing, peptides generated by cytosolic proteasomes are translocated by the transporter associated with antigen processing (TAP) into the endoplasmic reticulum, where they bind to newly synthesized MHC-I molecules. Dendritic cells and other cell types can also generate
Kolanjiyappan Vignesh et al.
Theriogenology, 149, 46-54 (2020-04-03)
Sub-fertility is a major problem in crossbred bulls. Identification of subtle differences in the quality of cryopreserved spermatozoa among bulls belonging to different fertility rankings would help determine the latent fertility of semen before their use at field conditions. In
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