N9287

Monoclonal Anti-NBS1 (Nibrin) antibody produced in mouse

~2 mg/mL, clone NBS1-501, purified immunoglobulin, buffered aqueous solution

• 동의어(들): Anti-Nijmegen Breakage Syndrome, Anti-p95 Protein of NBS1.MRE11/RAD50 Complex
• MDL number: MFCD03455620
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: NBS1-501, monoclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~95 kDa
• 종 반응성: human
• 농도: ~2 mg/mL
• 기술: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 2-4 μg/mL using nuclear extract of HEK 293T expressing recombinant human NBS1
• 동형: IgG1
• UniProt 수납 번호: O60934
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... NBN(4683)

설명

일반 설명

Monoclonal Anti-NBS1 (Nibrin) (mouse IgG1isotype) is derived from the hybridoma NBS1-501 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide of human NBS1. Nibrin is mapped to human chromosome 8q21.3. The encoded protein has two domains found in the cell cycle checkpoint proteins, forkhead-associated domain (FHA), and an adjacent breast cancer carboxy-terminal domain (BRCT).

면역원

synthetic peptide corresponding to amino acids 206-220 of human NBS1.

애플리케이션

Monoclonal Anti-NBS1 (Nibrin) antibody produced in mouse has been used in:

• antibody microarray
• immunocytochemistry
• immunoblotting
• immunoprecipitation
• enzyme linked immunosorbent assay (ELISA)

생화학적/생리학적 작용

NBS1(Nibrin) was first isolated as a protein involved in DNA repair through analysis of mutations in patients with this syndrome. p95/NBS1(Nibrin) deficiency abrogates the formation of the Meiotic recombination 11 homolog 1(MRE11) / DNA repair protein RAD50 ionizing radiation-induced foci, revealing a molecular link between double-stranded break repair (DSB) and cell cycle checkpoint functions. The phenotypic similarities between ataxia-telangiectasia (AT) and Nijmegen breakage syndrome had suggested that ataxia-telangiectasia mutated gene (ATM) and NBS1 functions in a common signaling pathway. This was confirmed by the finding that in response to ionizing radiation, NBS1is phosphorylated in Ser343 in an ataxia-telangiectasia mutated ATM-dependent manner.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable