N4662

Nek6, Active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE), buffered aqueous glycerol solution

• 동의어(들): Gene A-Related Kinase 6, NIMA-Related Kinase 6, Never in Mitosis
• MDL number: MFCD07370972
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 재조합: expressed in E. coli
• 분석: ≥80% (SDS-PAGE)
• 형태: buffered aqueous glycerol solution
• enzyme activity: ≥10 units/mg protein
• 분자량: protein apparent mol wt ~58 kDa
• UniProt 수납 번호: Q9HC98
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... NEK6(10783)

설명

애플리케이션

Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30 °C using a final concentration of 50 μM [³²P] ATP.

생화학적/생리학적 작용

Nek6 is a nuclear and cytoplasmic kinase that is required for mitotic progression of human cells. It is phosphorylated and activated during M phase of the cell cycle. Inhibition of Nek6 arrests cells in M phase and triggers apoptosis, suggesting that Nek6 is required for metaphase-anaphase transition.

단위 정의

One unit will incorporate 1 nanomole of phosphate into myelin basic protein (MBP) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm (32p) ATP

물리적 형태

Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable