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Merck
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Key Documents

N0913

Sigma-Aldrich

Anti-Neurocan antibody, Mouse monoclonal

clone 650.24, purified from hybridoma cell culture

동의어(들):

Anti-CSPG3, Anti-Chondroitin Sulfate Proteogycan 3

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

mouse

결합

unconjugated

항체 형태

purified from hybridoma cell culture

항체 생산 유형

primary antibodies

클론

650.24, monoclonal

형태

buffered aqueous solution

분자량

antigen 260 kDa (additional band at 160 kDa)

종 반응성

rat

포장

antibody small pack of 25 μL

농도

~1 mg/mL

기술

immunocytochemistry: suitable
immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
western blot: 0.1-0.2 μg/mL using neonatal rat brain treated with chondroitinase.

동형

IgG1

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

rat ... Ncan(58982)

일반 설명

Monoclonal Anti-Neurocan (mouse IgG1 isotype) is derived from the hybridoma 650.24 produced by the fusion of mouse myeloma cells (P3X cells) and splenocytes from BALB/c mice immunized with embryonic rat brain proteoglycans. Neurocan is a chondroitin sulfate proteoglycan (known also as CSPG3). It accounts for 20−30% of chondroitin sulfate proteoglycans in early postnatal rat brain. The mouse neurocan cDNA encodes for a 1,257-amino acid protein with a 22-amino acid signal peptide followed by an immunoglobulin-like domain and repeating motifs characteristic of the hyaluronic acid-binding region of aggregating proteoglycans. Neurocan is found in large amounts in glial scar, a primarily astrocytic structure formed after an injury in the central nervous system (CNS).
Neurocan is a proteoglycan which is expressed in the nervous tissue.

면역원

embryonic rat brain proteoglycans.

애플리케이션

Anti-Neurocan antibody, Mouse monoclonal has been used in:
  • western blotting
  • immunofluorescence
  • enzyme-linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • immunohistochemistry

생화학적/생리학적 작용

Neurocan can inhibit the growth of axons. In presence of central nervous system injuries, it also takes part in tissue repair and neural network reconstruction.
Neurocan participates in the modulation of cell adhesion and migration. It exerts a negative effect on growing cerebellar axons.

표적 설명

Neurocan is a chondroitin sulfate proteoglycan (knownalso as CSPG3) thought to be involved in the modulation of cell adhesion and migration. It accounts for 20-30% of chondroitin sulfate proteoglycans in early postnatal rat brain.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

AMPA Receptor Antagonist NBQX Decreased Seizures by Normalization of Perineuronal Nets
Chen W, et al.
PLoS ONE, 11(11), e0166672-e0166672 (2016)
Neurocan: a brain chondroitin sulfate proteoglycan
Rauch U, et al.
Cellular and Molecular Life Sciences, 58(12-13), 1842-1856 (2001)
Changes in neurocan expression in the distal spinal cord stump following complete cord transection: a comparison between infant and adult rats.
Qi ML
Neuroscience Research, 45(2), 181-188 (2003)
Characterization of the human neurocan gene, CSPG3
Prange CK, et al.
Gene, 221(2), 199-205 (1998)
Li Hong Shen et al.
Glia, 56(16), 1747-1754 (2008-07-12)
The glial scar, a primarily astrocytic structure bordering the infarct tissue inhibits axonal regeneration after stroke. Neurocan, an axonal extension inhibitory molecule, is up-regulated in the scar region after stroke. Bone marrow stromal cells (BMSCs) reduce the thickness of glial

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