추천 제품
사용
sufficient for 25 reactions
Quality Level
품질
Not suitable for clinical diagnostic use.
포장
pkg of 1 kit
저장 조건
dry at room temperature
기술
PCR: suitable
응용 분야
detection
microbiology
저장 온도
2-8°C
일반 설명
The LookOut Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals.
The primer/probe system detects the highly conserved 23S rRNA operon coding region of the mycoplasma genome. The kit is highly specific and does not detect eukaryotic DNA. The detection spectrum includes most mycoplasma species identified as cell culture contaminants (see Table 2 in the technical bulletin). The kit contains the nucleotide dUTP instead of dTTP and is, therefore, suitable for UNG pretreatment.
This kit already contains Taq polymerase in the Mycoplasma Mix.
The primer/probe system detects the highly conserved 23S rRNA operon coding region of the mycoplasma genome. The kit is highly specific and does not detect eukaryotic DNA. The detection spectrum includes most mycoplasma species identified as cell culture contaminants (see Table 2 in the technical bulletin). The kit contains the nucleotide dUTP instead of dTTP and is, therefore, suitable for UNG pretreatment.
This kit already contains Taq polymerase in the Mycoplasma Mix.
애플리케이션
qPCR Probe Kit for high quality and reliable quantitative detection of mycoplasma DNA in research, industrial application and product testing. Usable for direct testing of cell cultures and biologicals in combination with cell culture enrichment.
법적 정보
LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
Flash Point (°F)
188.6 °F
Flash Point (°C)
87 °C
문서
Know when to use antibiotics to prevent contamination in cell culture and find related products at Sigma-Aldrich.com
Detect mycoplasma contamination in cell cultures three ways: culture test, DNA stain test, or mycoplasma PCR test. Mycoplasma elimination kits rid cultures of contamination.
프로토콜
Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.
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