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Key Documents

MP0040A

Sigma-Aldrich

LookOut® Mycoplasma qPCR Detection Kit

동의어(들):

Quantiative PCR mycoplasma detection kit

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About This Item

EC Number:
UNSPSC 코드:
12352207
NACRES:
NA.75

사용

sufficient for 25 reactions

Quality Level

품질

Not suitable for clinical diagnostic use.

포장

pkg of 1 kit

저장 조건

dry at room temperature

기술

PCR: suitable

응용 분야

detection
microbiology

저장 온도

2-8°C

일반 설명

The LookOut Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals.

The primer/probe system detects the highly conserved 23S rRNA operon coding region of the mycoplasma genome. The kit is highly specific and does not detect eukaryotic DNA. The detection spectrum includes most mycoplasma species identified as cell culture contaminants (see Table 2 in the technical bulletin). The kit contains the nucleotide dUTP instead of dTTP and is, therefore, suitable for UNG pretreatment.

This kit already contains Taq polymerase in the Mycoplasma Mix.

애플리케이션

qPCR Probe Kit for high quality and reliable quantitative detection of mycoplasma DNA in research, industrial application and product testing. Usable for direct testing of cell cultures and biologicals in combination with cell culture enrichment.

법적 정보

LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

Flash Point (°F)

188.6 °F

Flash Point (°C)

87 °C


시험 성적서(COA)

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문서 라이브러리 방문

문서

Know when to use antibiotics to prevent contamination in cell culture and find related products at Sigma-Aldrich.com

Detect mycoplasma contamination in cell cultures three ways: culture test, DNA stain test, or mycoplasma PCR test. Mycoplasma elimination kits rid cultures of contamination.

프로토콜

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

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