추천 제품
사용
kit sufficient for 25 tests
Quality Level
포장
pkg of 1 kit
저장 조건
dry at room temperature
기술
PCR: suitable
응용 분야
detection
microbiology
저장 온도
2-8°C
일반 설명
The Venor™ GeM Mycoplasma Detection Kit utilizes the polymerase chain reaction (PCR), which was established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals. Detection requires as little as 1–5fg of mycoplasma DNA corresponding to 2–5 mycoplasma per sample volume. The primer set is specific to the highly conserved rRNA operon, or more specifically, the 16S rRNA coding region in the mycoplasma genome. This allows for detection of all Mycoplasma, Acholeplasma and Ureaplasma species tested so far, which are usually encountered as contaminants in cell cultures. Eukaryotic and bacterial DNA are not amplified by this kit.
애플리케이션
The PCR-based Venor™ GeM Mycoplasma Detection Kit employs PCR technology for rapid and reliable detection of mycoplasma DNA in cell cultures and virus stocks.
성분
Does not include Taq Polymerase. Optimized for use with D9307, Taq DNA Polymerase
저장 및 안정성
The kit components are stable during shipping at ambient temperature. Upon receipt, store at 2–8 °C. After rehydration of the primer/nucleotide mix, the positive control, and the internal control, store below –20 °C and avoid repeated freezing and thawing. For repeated testing of low sample numbers, primer/nucleotide mix and controls should be aliquoted after rehydration. By following these recommendations, the kit is stable until the expiration date stated on the label.
법적 정보
Venor is a trademark of Minerva Biolabs GmbH
키트 구성품 전용
제품 번호
설명
- Positive Control
- Negative Control
- PCR 10X Reaction Buffer 500 mL/vial
- Primer/Nucleotide Mix 1 mL/vial
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
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문서
Know when to use antibiotics to prevent contamination in cell culture and find related products at Sigma-Aldrich.com
Detect mycoplasma contamination in cell cultures three ways: culture test, DNA stain test, or mycoplasma PCR test. Mycoplasma elimination kits rid cultures of contamination.
프로토콜
Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.
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