콘텐츠로 건너뛰기
Merck
모든 사진(1)

Key Documents

MAK332

Sigma-Aldrich

Pyruvate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

동의어(들):

Pyruvic Acid Detection Kit

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

사용

sufficient for 100 colorimetric or fluorometric tests

입력

biological sample(s)

응용 분야

pharmaceutical

검출 방법

colorimetric
fluorometric

관련 질환(들)

gastrointestinal diseases; cancer

저장 온도

−20°C

일반 설명

Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery.

애플리케이션

The Pyruvate Assay Kit may be used for:
  • Drug Discovery
  • Food and Beverage Testing
  • Neurological Disease Research

특징 및 장점

Broad Detection Range: Detect Pyruvate levels accurately across a wide range, from 2 µM to 500 µM, using only 10 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.



Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 30 minute room temperature reaction, reducing complexity and saving valuable time and effort.



Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

적합성

Suitable for pyruvate detection in biological samples and for studying the effects of drugs on pyruvate metabolism.

원리

The Pyruvate Assay Kit uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λem/ex = 585/530nm is directly proportional to pyruvate concentration in the sample. Linear detection range in 96-well plate: 2 to 500 μM (17 μg/dL to 4.4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 μM for fluorometric assays.

기타 정보

For additional information on our range of Biochemicals, please complete this form.

Storage Class Code

10 - Combustible liquids

Flash Point (°F)

188.6 °F

Flash Point (°C)

87 °C


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.