M9445

Matrix Metalloproteinase-2 from mouse

recombinant, expressed in NSO cells, >95% (SDS-PAGE), buffered aqueous glycerol solution

• 동의어(들): Collagenase Type IV 72 kDa, Gelatinase 72 kDa, Gelatinase A, MMP-2
• MDL number: MFCD01324322
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 재조합: expressed in NSO cells
• Quality Level: 200
• 분석: >95% (SDS-PAGE)
• 형태: buffered aqueous glycerol solution
• 분자량: apparent mol wt ~72 kDa
• UniProt 수납 번호: P33434; P33436
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: mouse ... Mmp2(17390) ; rat ... Mmp2(81686)

설명

일반 설명

Matrix Metalloproteinase-2 (MMP-2) also known as gelatinase or type IV collagenase is a 72kDa protein. MMP-2 is a member of matrix metalloproteinase (MMP) family of enzymes. Basic structure of MMP2 contains signal peptide domain that targets the enzyme for secretion, the pro-peptide domain, which is removed when the enzyme is activated and the catalytic site containing gelatin-binding domain.

특이성

The amino acid sequences 1-662 of the proenzymes of MMP-2 are identical between mouse and rat.

애플리케이션

Matrix metalloproteinase-2 (MMP2) human has been used as a standard in zymography to measure proteolytic activity of MMP-2.

생화학적/생리학적 작용

Matrix Metalloproteinase-2 (MMP-2) cleaves gelatin, type IV, V, VII, X, and XI collagens, fibronectin, elastin, laminin, proteoglycans and a range of non extracellular matrix (ECM ) components. MMP-2 cleaves native type I collagen to N-terminal ¾ and C-terminal ¼ fragments identical to those generated by interstitial collagenases. MMP2 and MMP9 play an essential role in matrix degradation and they are implicated in the maintenance of neovascularization. In mice, deletion or inhibition of MMP2 protects against myocardial rupture.

MMP-2 degrades general matrix components and may have a role in processes such as host defense, cell proliferation, and protein turnover as well as tissue remodeling.

물리적 형태

Supplied as a 0.2 μm filtered solution of 25 mM Tris, pH 7.5, 5 mM calcium chloride, 75 mM sodium chloride, 0.025% Brij^® 35 and 50% glycerol.

분석 메모

The biological activity is measured by its ability to cleave a fluorogenic peptide sustrate.

법적 정보

Brij is a registered trademark of Croda International PLC

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)