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Merck
모든 사진(1)

주요 문서

M8645

Sigma-Aldrich

Anti-Mouse IgG (whole molecule) antibody produced in goat

affinity isolated antibody, lyophilized powder

동의어(들):

Goat Anti-Mouse IgG, Goat IgG Antibody

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

goat

Quality Level

결합

unconjugated

항체 형태

affinity isolated antibody

항체 생산 유형

secondary antibodies

클론

polyclonal

양식

lyophilized powder

기술

Ouchterlony double diffusion: suitable

저장 온도

2-8°C

타겟 번역 후 변형

unmodified

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일반 설명

IgG is the main antibody type found in plasma and extracellular fluid and is expressed on B cell membrane.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

면역원

IgG purified from pooled normal mouse serum.

애플리케이션

Anti-mouse IgG (whole molecule) antibody (diluted 1:20) can be used in Indirect immunofluorescence. It can also be used as a secondary antibody in western blotting to release NC1 domain of type IV collagen. Further, it can also be used in enzyme immunoassays (50 μgram) .
Anti-mouse IgG (whole molecule) antibody (diluted 1:20) can be used in:
  • indirect immunofluorescence
  • western blotting to release NC1 domain of type IV collagen
  • enzyme immunoassays (50 μgram)

생화학적/생리학적 작용

IgG regulates several functions such as phagocytosis and hence plays a pivotal role in facilitating cytological immune responses. Anti-mouse IgG (whole molecule) antibody can be used in T cell proliferation assays. It can also be used in Ouchterlony Double Diffusion and immunoelectrophoresis. Goat anti- mouse IgG (whole molecule) antibody reacts specifically with mouse IgG subclasses IgG1, IgG2a, IgG2b and IgG3. The product has also shown no reactivity to human serum proteins.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

기타 정보

Antibody adsorbed with human serum proteins

물리적 형태

Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2

재구성

Reconstitute with 1 mL of 0.135 M sodium chloride.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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문서 라이브러리 방문

E M Rust et al.
The Journal of clinical investigation, 103(10), 1459-1467 (1999-05-20)
The direct effects of expressing hypertrophic cardiomyopathy-associated (HCM-associated) mutant troponin T (TnT) proteins on the force generation of single adult cardiac myocytes have not been established. Replication-defective recombinant adenovirus vectors were generated for gene transfer of HCM-associated I79N and R92Q
T Keeley et al.
Zoo biology, 31(3), 275-290 (2011-03-16)
The Vancouver Island marmot (Marmota vancouverensis; VIM) is one of North America's most endangered species with fewer than 150 individuals remaining in the wild. A captive breeding program was established across four facilities in Canada as an insurance population and
Molecular Genetics of Immunoglobulin, 17 (1987)
The Laboratory Rat (2005)
C S von Bartheld et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 20(2), 736-748 (2000-01-13)
Anterograde axonal transport of neurotrophins has been demonstrated recently, but to date such transport has only been shown for brain-derived neurotrophic factor and no other endogenous neurotrophin. Endogenous neurotrophin-3 (NT-3) protein is present in the ganglion cell layer of the

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