H9539

Anti-HMGB1 (HMG1) (C-terminal) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• 동의어(들): Anti-Amphoterin, Anti-High mobility group box 1, Anti-High mobility group protein 1
• MDL number: MFCD02263062
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: buffered aqueous solution
• 분자량: antigen 25 kDa
• 종 반응성: rat, mouse, human
• 포장: antibody small pack of 25 μL
• 향상된 검증: independent; Learn more about Antibody Enhanced Validation
• 기술: immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates.; indirect immunofluorescence: suitable; western blot: 0.5-1 μg/mL using 3T3 or PC12 cell lysates.
• UniProt 수납 번호: P09429
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... HMGB1(3146) ; mouse ... Hmgb1(15289) ; rat ... Hmgb1(25459)

설명

일반 설명

The High Mobility Group (HMG1) is secreted by activated monocytes and macrophages. The gene is mapped to human chromosome 13q12.

면역원

synthetic peptide corresponding to amino acid 165-180 of human HMGB1, conjugated to KLH. The corresponding sequence is conserved in mouse and rat.

애플리케이션

Anti-HMGB1 (HMG1) (C-terminal) antibody produced in rabbit has been used in ELISA and immunofluorescence.

생화학적/생리학적 작용

In the nucleus, HMGB1 (high-mobility group protein B1) works as a nucleosome stabilizer and transcriptional regulator. It also exhibits chaperone-like activity and suppresses aggregation of polyglutamine. It has a role in inflammation and autophagy. HMGB1 can interact with toll-like receptor (TLR) 2 and 4, interleukin-1β, chemokine (C-X-C motif) ligand 12 (CXCL12) and pathogen-associated molecular pattern (PAMP) molecules. It also works as a damage-associated molecular pattern molecule (DAMP).

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

저장 및 안정성

For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots.
Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

기타 정보

In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working dilutions by titration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)