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H9394

Sigma-Aldrich

Hanks′ Balanced Salt solution

Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

동의어(들):

HBSS

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.75

Quality Level

400

무균

sterile-filtered

형태

liquid

기술

cell culture | mammalian: suitable

불순물

endotoxin, tested

성분

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.011 g/L

배송 상태

ambient

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Hanks′ Balanced Salt solution is recommended for suspension cultures or whenever cell clumping is a problem.

애플리케이션

Hanks′ Balanced Salt solution has been used:
  • as a component of the culture medium for culturing of mouse cortical slices
  • as a component of the dissociation buffer for incubation of human-induced pluripotent stem cells (hiPSCs) and hiPSC-derived cardiomyocytes (CMs)
  • for in situ perfusions of mice liver

생화학적/생리학적 작용

Balanced salt solution in cell culture plays multiple roles such as, an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance, and provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. It is also involved in providing a buffering system to maintain the medium within the physiological pH range (7.2-7.6) and provides the principal energy source for cell metabolism.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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프로토콜

Removal of Adherent Cells from a Culture Surface Using Trypsin

Trypsin may be used to remove adherent cells from a culture surface. Cells are most commonly removed from the culture substrate by treatment with trypsin or trypsin/EDTA solutions.

StableCell™ Trypsin Solutions

StableCell™ Trypsin solutions are designed to perform cell detachment as standard trypsin solutions do, without the need to aliquot, freeze, and thaw. This saves significant time before passaging.

StableCell™ Trypsin Solutions

StableCell™ Trypsin solutions are designed to perform cell detachment as standard trypsin solutions do, without the need to aliquot, freeze, and thaw. This saves significant time before passaging.

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