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Merck
모든 사진(1)

Key Documents

GE27-4577-01

Sigma-Aldrich

Anti-GST Antibody

Cytiva 27-4577-01

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About This Item

UNSPSC 코드:
12352116
NACRES:
NA.32

결합

unconjugated

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

제조업체/상표

Cytiva 27-4577-01

포장

pkg of 0.5 mL, 50 detections

배송 상태

wet ice

저장 온도

2-8°C

관련 카테고리

일반 설명

Anti-GST Antibody is a polyclonal antibody purified from the sera of goats immunized with purified schistosomal GST. Because of its polyclonal nature, it can recognize more than one epitope on GST, thereby improving its capacity for recognizing GST fusion proteins even if some binding sites are masked due to recombinant protein folding.

애플리케이션

Western Blots

특징 및 장점

  • For the highly sensitive and specific detection of recombinant schistosomal GST fusion proteins expressed using pGEX vectors
  • Extensively cross-adsorbed to remove antibodies that bind to E. coli proteins and affinity purified using Glutathione Sepharose
  • Supplied unconjugated for use with any enzyme-conjugated anti-goat antibody
  • Recommended for use in Western blots and dot blots to detect GST fusion proteins

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

GST Gene Fusion Vectors
A license for commercial use of GST Gene Fusion Vectors under US patent 5,654,176 and equivalent patents and patent applications in other countries must be obtained from Millipore Corp (formerly Chemicon International Inc).
Sepharose is a trademark of Cytiva

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Storage Class Code

12 - Non Combustible Liquids


시험 성적서(COA)

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문서 라이브러리 방문

Claire E J Moore et al.
Cellular signalling, 28(4), 284-293 (2016-01-23)
Eukaryotic elongation factor 2 kinase (eEF2K) inhibits the elongation stage of protein synthesis by phosphorylating its only known substrate, eEF2. eEF2K is tightly regulated by nutrient-sensitive signalling pathways. For example, it is inhibited by signalling through mammalian target of rapamycin
Apoorva Baluapuri et al.
Molecular cell, 74(4), 674-687 (2019-04-01)
The MYC oncoprotein binds to promoter-proximal regions of virtually all transcribed genes and enhances RNA polymerase II (Pol II) function, but its precise mode of action is poorly understood. Using mass spectrometry of both MYC and Pol II complexes, we

문서

Troubleshooting of Detection Methods of GST-tagged Proteins

프로토콜

This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from GE Healthcare.

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