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특징
autoclavable In wet form (pH 7.0) at 120°C for 30 min
포장
pack of 100 g
제조업체/상표
Cytiva 17-0050-01
파라미터
flow rate (The beads behave as rigid spheres obeying Darcy′s Law.)
flow rate
Matrix
cross-linked dextran
입자 크기
40-120 μm (dry)
90-280 μm (wet)
작동 범위
2-10
일반 설명
Sephadex® G-75 is well established gel filtration medium for desalting and buffer exchange of large biomolecules >80 000 molecular weight.
Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.
Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.
특징 및 장점
- Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
- Suitable for buffer exchange and cleanup of large biomolecules, >80 000 molecular weight
- Highest exclusion limit, 80 000, in the Sephadex® G-range
- Classic gel filtration medium.
저장 및 안정성
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()
분석 메모
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
법적 정보
Sephadex is a registered trademark of Cytiva
Storage Class Code
13 - Non Combustible Solids
가장 최신 버전 중 하나를 선택하세요:
프로토콜
This page shows how to perform a separation with a Sephadex column from GE Healthcare which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.
관련 콘텐츠
Separation options for Size Exclusion Chromatography with Sephadex from Cytiva.
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