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Key Documents

GE17-0043-02

Sephadex® G-50

Medium, Cytiva, pack of 500 g

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About This Item

CAS Number:
MDL number:
UNSPSC 코드:
41115722
NACRES:
NA.56

특징

autoclavable In wet form (pH 7.0) at 120°C for 30 min

포장

pack of 500 g

제조업체/상표

Cytiva

팽창

1 g swells to 9-11 mL

입자 크기

100-300 μm (wet)
50-150 μm (dry)

cleaning in place

2-13

working range

2-10

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

InChI key

FZWBNHMXJMCXLU-UHFFFAOYSA-N

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관련 카테고리

일반 설명

Sephadex® G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations.

Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

특징 및 장점

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Suitable for purification of DNA from small molecules by gel filtration
  • Larger bead size for large scale group separations where high flow rates and low operating pressures are required.
  • Classic gel filtration medium.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

Sephadex is a registered trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids


시험 성적서(COA)

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프로토콜

This page shows how to perform a separation with a Sephadex column from GE Healthcare which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.

관련 콘텐츠

Sephadex® from Cytiva and Darcy’s law in Size Exclusion Chromatography

Separation options for Size Exclusion Chromatography with Sephadex from Cytiva.

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