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Merck
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Key Documents

G8021

Sigma-Aldrich

Monoclonal Anti-β-Galactosidase antibody produced in mouse

clone GAL-13, ascites fluid

동의어(들):

Monoclonal Anti-β-Galactosidase

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

mouse

Quality Level

결합

unconjugated

항체 형태

ascites fluid

항체 생산 유형

primary antibodies

클론

GAL-13, monoclonal

포함

15 mM sodium azide

종 반응성

bacteria

기술

dot blot: suitable using soluble β-galactosidase
immunocytochemistry: suitable
indirect ELISA: 1:2,000 using mouse primary antibody, bridging antibody and Escherichia coli β-galactosidase

동형

IgG1

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

Escherichia coli ... lacZ(1036228) , lacZ(1036228)
Escherichia coli K12 ... Lacz(945006)

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Monoclonal Anti-β-Galactosidase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. β −galactosidase is a E coli protein and is a tetramer with 1023 amino acids.

특이성

The antibody may be used for amplification in immunoenzymatic staining by preparing a β-galactosidase anti-β-galactosidase (BGABG) soluble complex. It is not recommended for immunoblotting; it does not recognize denatured or reduced β-galactosidase.

면역원

β-galactosidase from E. coli

애플리케이션

Monoclonal Anti-β-Galactosidase antibody produced in mouse has been used in:

  • immunostaining and histochemistry
  • immunostaining and microscopy
  • immunohistochemistry
  • immunofluorescence staining
  • enzyme linked immunosorbent assay(ELISA)
  • immuno dot blot

생화학적/생리학적 작용

β −galactosidase substrate is lactose. Synthetic substrates like o-nitrophenyl-β-d-galactopyranoside (oNPG) or p-nitrophenyl-β-d-galactopyranoside (pNPG) are used in enzymatic assays. It is also used as marker in studying gene expression. The use of monoclonal β -galactosidase anti- β -galactosidase (BGABG) soluble complex in the unlabeled antibody-enzyme method results in an intense signal with a very low background.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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