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Merck
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Key Documents

G7402

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)–Gold antibody produced in goat

affinity isolated antibody, aqueous glycerol suspension, 10 nm (colloidal gold)

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

goat

결합

gold conjugate

항체 형태

affinity isolated antibody

항체 생산 유형

secondary antibodies

클론

polyclonal

형태

aqueous glycerol suspension

기술

dot blot: suitable
western blot: suitable

입자 크기

10 nm (colloidal gold)

저장 온도

2-8°C

타겟 번역 후 변형

unmodified

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

IgG antibody plays a crucial role in humoral immune responses such as complement activation, phagocytosis, placental transport and cell surface-receptor binding. Anti-rabbit IgG (whole molecule)-gold antibody can be used as secondary antibody (diluted 1:50 in 0.01 M PBS containing 1% BSA and 5% NGS) in EM immunolabeling of GABA and glutamate by postembedding procedure. It can also be used in a modified dot blot assay to determine binding activity. Goat anti-rabbit IgG (whole molecule)-gold antibody reacts specifically with all goat immunoglobulins.
Immunoglobulins (Igs) are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs can be useful tools for the analysis of target proteins
Anti-Rabbit IgG (whole molecule)-Gold antibody is specific for rabbit IgG.

면역원

Rabbit IgG purified from normal rabbit serum.

애플리케이션

Anti-rabbit IgG (whole molecule)-gold antibody (diluted 1: 15) can be used in immunoelectron microscopy. It can also be used in western blotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immuno-electron microscopy (1 paper)
Immunohistochemistry (1 paper)

물리적 형태

Colloidal suspension in Tris buffered saline, pH 8.2, with 30% glycerol (v/v), 1% bovine serum albumin (w/v), and 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


시험 성적서(COA)

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이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Ignaz Wessler et al.
PloS one, 11(6), e0156886-e0156886 (2016-06-11)
The worldwide use of neonicotinoid pesticides has caused concern on account of their involvement in the decline of bee populations, which are key pollinators in most ecosystems. Here we describe a role of non-neuronal acetylcholine (ACh) for breeding of Apis
S Sinnarajah et al.
FEBS letters, 426(3), 377-380 (1998-05-26)
The odorant-induced accumulation of cAMP can be inhibited by antibodies directed against G alpha s/olf. In contrast, antibodies raised against G alpha i-subunits caused a strong enhancement of the odorant-induced cAMP accumulation. Western blotting and immunoelectron microscopy revealed the presence
Andrea González et al.
The American journal of tropical medicine and hygiene, 92(5), 887-897 (2015-03-12)
Trypanosoma cruzi calreticulin (TcCRT), a 47-kDa chaperone, translocates from the endoplasmic reticulum to the area of flagellum emergence. There, it binds to complement components C1 and mannan-binding lectin (MBL), thus acting as a main virulence factor, and inhibits the classical
Jiongjiong Chen et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(32), 11436-11441 (2008-08-06)
Hybrid sterility is a major form of postzygotic reproductive isolation. Although reproductive isolation has been a key issue in evolutionary biology for many decades in a wide range of organisms, only very recently a few genes for reproductive isolation were
Vladimir Varga et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(32), E6546-E6555 (2017-07-21)
The distal end of the eukaryotic flagellum/cilium is important for axonemal growth and signaling and has distinct biomechanical properties. Specific flagellum tip structures exist, yet their composition, dynamics, and functions are largely unknown. We used biochemical approaches to identify seven

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