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Merck
모든 사진(1)

주요 문서

G5518

Sigma-Aldrich

Anti-Goat IgG (whole molecule) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

rabbit

Quality Level

결합

unconjugated

항체 형태

IgG fraction of antiserum

항체 생산 유형

secondary antibodies

클론

polyclonal

양식

buffered aqueous solution

기술

indirect ELISA: 1:50,000
quantitative precipitin assay: 3.5 mg/mL

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

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일반 설명

IgG is a glycoprotein. It is a major class of immunoglobulin. IgG consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which is linked together by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4.

면역원

Anti-Goat IgG is produced in rabbit using as immunogen purified goat IgG.

애플리케이션

Anti-Goat IgG (whole molecule) antibody produced in rabbit has been used in chromatin immunoprecipitation (ChIP) assay and electron microscopy.
Anti-Goat IgG (whole molecule) antibody produced in rabbit was used in combination with protein A-colloidal gold conjugate in electron microscopy of in vitro matured and fertilized boar oocytes.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunoprecipitation (1 paper)

생화학적/생리학적 작용

IgG antibody subtype is the most abundant serum immunoglobulin of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative

저장 및 안정성

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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Lot/Batch Number

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이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

The primate-specific microRNA gene cluster (C19MC) is imprinted in the placenta
Noguer-DM, et al.
Human Molecular Genetics, 19(18), 3566-3582 (2010)
The Immunoglobulins: Structure and Function (1987)
Different BCR/Abl protein suppression patterns as a converging trait of chronic myeloid leukemia cell adaptation to energy restriction
Bono S, et al.
Testing, 7(51), 84810-84810 (2016)
Stefania Cannito et al.
Oncotarget, 6(4), 2206-2221 (2014-12-30)
SERPINB3 is a cysteine-proteases inhibitor up-regulated in a significant number of cirrhotic patients carrying hepatocellular carcinoma (HCC) and recently proposed as a prognostic marker for HCC early recurrence. SERPINB3 has been reported to stimulate proliferation, inhibit apoptosis and, similar to
Victor Crivianu-Gaita et al.
Biochemistry and biophysics reports, 2, 23-28 (2015-04-25)
Antibodies have widespread applications in areas ranging from therapeutics to chromatography and protein microarrays. Certain applications require only the fragment antigen-binding (Fab) units of the protein. This study compares the cleavage efficacy of dithiothreitol (DTT), mercaptoethylamine (MEA), and dithiobutylamine (DTBA)

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