G211150

DMT-2′O-Methyl-rG(ib) Phosphoramidite

configured for (ÄKTA^® and OligoPilot^®)

• 동의어(들): DMT-2′-O-Me-rG(ib) amidite, N-(2-methyl-1-oxopropyl)-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-O-methyl-guanosine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite]
• 실험식(Hill 표기법): C₄₅H₅₆N₇O₉P
• CAS Number: 150780-67-9
• Molecular Weight: 869.94
• MDL number: MFCD00792625
• UNSPSC 코드: 41116105
• PubChem Substance ID: 329799866
• NACRES: NA.51

속성

• 생물학적 소스: non-animal source (no BSE/TSE risk)
• Quality Level: 300
• 제품 라인: Proligo Reagents
• 분석: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• 양식: powder
• 기술: oligo synthesis: suitable
• 불순물: ≤0.1% single unspecified Impurity (reversed phase HPLC); ≤0.3% mG2 (reversed phase HPLC, Hydrolysate); ≤0.3% mG3 (reversed phase HPLC, DMT-rG(ib)me); ≤0.3% water content (Karl Fischer); ≤0.5% P(III) Impurities 100-169ppm (³¹P-NMR); ≤1.0% mG1 (reversed phase HPLC, DMT-rG(ib)me-DMT); ≤3% residual Solvent content
• 색상: white to off-white
• λ: conforms (UV/VIS Identity)
• 적합성: conforms to structure for H-NMR; conforms to structure for LC-MS
• 호환성: configured for (ÄKTA^® and OligoPilot^®)
• 뉴클레오시드 프로파일: base: guanosine; base protecting group: isobutyryl; 2' protecting group: methyl; 5' protecting group: DMT; deprotection: standard
• 저장 온도: 2-8°C
• SMILES string: CO[C@@H]1[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(c2ccccc2)(c3ccc(OC)cc3)c4ccc(OC)cc4)O[C@H]1n5cnc6C(=O)NC(NC(=O)C(C)C)=Nc56
• InChI: 1S/C45H56N7O9P/c1-28(2)41(53)49-44-48-40-37(42(54)50-44)47-27-51(40)43-39(57-9)38(61-62(59-25-13-24-46)52(29(3)4)30(5)6)36(60-43)26-58-45(31-14-11-10-12-15-31,32-16-20-34(55-7)21-17-32)33-18-22-35(56-8)23-19-33/h10-12,14-23,27-30,36,38-39,43H,13,25-26H2,1-9H3,(H2,48,49,50,53,54)/t36-,38-,39-,43-,62?/m1/s1
• InChI key: IRRDHRZUOZNWDJ-MLLDKZSOSA-N

설명

일반 설명

Proligo′s 2′O-Methyl RNA monomers are compatible with fast deprotectionschemes that are based on the application of aliphatic amines, such asmethylamine. The adenosine and guanosine monomers are protected bythe standard benzoyl (bz) and isobutyryl (ib) groups.2′O-Methyl RNA is a nucleic acid analog that is characterized by theexceptional hybridization properties that it imparts with complimentaryDNA or RNA, as well as increased stability against enzymatic degradationcompared to natural nucleic acids.The unique combination of properties of 2′O-Methyl RNA had foundwidespread use in the fields of:

• Diagnostic probes
• Aptamer and ribozyme development
• Mixed 2′O-Methyl-RNA/DNA antisense molecules

Key Features:

• High yield of crude oligonucleotides
• Compatible with DNA synthesis
• Can be employed together with DNA or RNA phosphoramidites in thesame synthesis to produce mixmer oligonucleotides
• Recommended deprotection conditions are 8 hours at 55 °C usingconcentrated ammonia solution, or with AMA (concentrated ammonia/40% aqueous methylamine I/I, v/v) for 10 minutes at 65 °C
• Purification and other downstream processing of fully modified 2′OMethyl RNA oligonucleotides are simpler than in the case of RNA, as nospecial precautions are required to provide protection against nucleolyticdegradationDMT-2′O-Methyl-rG(ib)

Phosphoramidite is configured for ÄKTA® Synthesizers.

법적 정보

OligoPilot is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable