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Merck
모든 사진(1)

주요 문서

F4639

Sigma-Aldrich

Monoclonal Anti-Fibrinogen antibody produced in mouse

clone FG-21, ascites fluid

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

mouse

결합

unconjugated

항체 형태

ascites fluid

항체 생산 유형

primary antibodies

클론

FG-21, monoclonal

포함

15 mM sodium azide

종 반응성

human, baboon

기술

indirect ELISA: 1:4,000

동형

IgG2a

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

일반 설명

Fibrinogen, is a thrombin-coagulable soluble plasma 340kDa glycoprotein, composed of paired sets of three subunits i.e. α, β, γ. Among three subunits, β and γ subunits contain one N-glycosylation site, which is occupied by a biantennary N-glycan. It contains three pairs of disulfide bonded chains called α, β, γ which further folded into four structural domains: the D, E, connector and the COOH-terminal region of the Aα chain.
Monoclonal Anti-Fibrinogen (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

특이성

The antibody is specific for human fibrinogen and recognizes an epitope sensitive to reduction commonly used in immunoblotting assays. It also reacts with synthetic human fibrinopeptides A and B, fibrinogen related peptides and baboon fibrinogen. No cross-reactivity with bovine, pig, sheep, horse, dog, goat, rabbit, guinea pig, or rat is observed.

면역원

purified haptoglobin from pooled human plasma containing the three major haptotypes.

애플리케이션

Monoclonal Anti-Fibrinogen antibody produced in mouse has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • fibrinogen adsorption assay

생화학적/생리학적 작용

Fibrinogen, a blood coagulation protein, is regarded as the central protein in the blood coagulation system.Fibrinogen plays a crucial role in protecting the vascular network against the loss of blood after tissue injury.
Plasmin attacks the Aα chain COOH domain to produce the heterogeneous fragment X. After a series of degradation, it ended with terminal digestion products: fragments D and E which represent the major globular domains in fibrinogen. Mutations in this gene leads to several disorders including hypofibrinogenemia, afibrinogenemia.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

Preservation of hematopoietic stem and progenitor cells from umbilical cord blood stored in a surface derivatized with polymer nanosegments
Higuchi A, et al.
Biomacromolecules, 9(2), 634-639 (2008)
Akon Higuchi et al.
Biomaterials, 24(19), 3235-3245 (2003-05-24)
We examined plasma protein adsorption and platelet adhesion to polysulfone (PSf) flat membranes coated with Pluronic with varying polyethylene oxide (PEO) block length. Adsorption of albumin, globulin and fibrinogen to Pluronic-coated PSf membranes was independent of plasma dilution when concentrations
Identification of Apo-A1 as a biomarker for early diagnosis of bladder transitional cell carcinoma
Li H, et al.
Proteome Science, 9(1), 21-21 (2011)
N E Kirschbaum et al.
The Journal of biological chemistry, 265(23), 13669-13676 (1990-08-15)
The COOH-terminal portion of the A alpha chain of human fibrinogen is highly susceptible to proteolytic degradation. This property has prevented isolation of the COOH-terminal domain of fibrinogen for the direct investigation of its functional characteristics. Human fibrinogen was degraded
Y Fang et al.
Haemophilia : the official journal of the World Federation of Hemophilia, 12(6), 615-620 (2006-11-07)
Congenital afibrinogenaemia is a rare autosomal recessive disorder, characterized by the complete absence or extremely reduced level of fibrinogen (Fg). We attempted to analyse the phenotype and genotype in two Chinese families with congenital afibrinogenaemia. Coagulation studies including activated partial

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