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Merck
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Key Documents

F1773

Sigma-Aldrich

Anti-CD4−FITC antibody, Mouse monoclonal

clone Q4120, purified from hybridoma cell culture

동의어(들):

Monoclonal Anti-CD4

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.44

생물학적 소스

mouse

결합

FITC conjugate

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

Q4120, monoclonal

형태

buffered aqueous solution

분자량

antigen 59 kDa

종 반응성

human

기술

flow cytometry: 10 μL using 1 × 106 cells

동형

IgG1

UniProt 수납 번호

배송 상태

wet ice

저장 온도

2-8°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... CD4(920)

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Anti-CD4-FITC antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from Balb/c mice immunized with cluster of differentiation 4 (CD4)-transfected mouse T cell hybridoma, 3DT, followed by CD4+ human T cell CEM cells (leukemic lymphoblasts).
CD4, a glycoprotein, is predominantly expressed on the cell membrane of mature, thymus-derived (T) lymphocyte and also to some extent in the monocyte/macrophage lineage. It has a transmembrane domain, cytoplasmic region and an extracellular region of four tandem domain similar like members of Ig superfamily.

특이성

Recognizes the CD4. The epitope recognized by the Q4120 clone is located on 1-183 aa and is sensitive to formalin fixation and paraffin embedding. 5th Workshop: code no. CD04.11

면역원

CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.

애플리케이션

FITC Conjugated Monoclonal Anti-Human CD4 has been used for:
  • identification, quantification and monitoring of helper/inducer T cells in peripheral blood, biological fluids, lymphoid organs, and other tissues
  • analysis of T cell mediated cytotoxicity
  • characterization of subtypes of T cell leukemias and lymphomas
  • studies of T cells in health and disease
  • flow cytometry

생화학적/생리학적 작용

CD4+ T cells acts as a helper/inducer, in providing an activating signal to B cells. It induces T lymphocytes bearing the reciprocal CD8 marker to become cytotoxic/suppressor cells. It is also majorly associated with non-polymorphic region of MHC (major histocompatibility complex) antigen in interacting with targets bearing MHC class II molecules. Thus, it is called as an adhesion molecule. It also behaves as receptor for the GP 120 envelope glycoprotein of human immunodeficiency virus. The CD4 molecules is involved in the adhesion of T lymphocytes to target cells, thymic development, transmission of intracellular signals during T cell activation, and binding to polyclonal immunoglobulins.
Immunoregulatory T cell subset abnormalities in autoimmunity disorders, immunodeficiency diseases, graft versus host disease and following immunosuppressive therapy are often manifested as a change in CD4+/CD8+ ratio in peripheral blood T cells.

표적 설명

CD4 is a single chain transmembraneous glycoprotein from the immunoglobulin superfamily. It is expressed on the helper/inducer T subset, on most medullary thymocytes, on microglial, dendritic and on some malignancies of T cell origin. The antigen binds to MHC class II molecules and is associated with p56lck protein tyrosine kinase.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

제조 메모

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

The production of armed effector T cells
Janeway, et al.
Immunobiology (2001)
Qi-Zhi Zhang et al.
Molecular medicine reports, 22(5), 3629-3634 (2020-10-02)
Phosphoinositide 3-kinase catalytic subunit δ isoform (P110δ) is mainly expressed in white blood cells. It is involved in T and B lymphocyte differentiation, maturation and the neutrophil chemotaxis process. Apolipoprotein E (ApoE) is an arginine‑rich alkaline protein, which is present
Variation of blood T lymphocyte subgroups in patients with non-small cell lung cancer
Wang WJ, et al.
Asian Pacific Journal of Cancer Prevention, 14(8), 4671-4673 (2013)
Ravi Tandon et al.
AIDS research and human retroviruses, 30(7), 654-664 (2014-05-03)
Galectin-9 (Gal-9) is a β-galactosidase-binding lectin that promotes apoptosis, tissue inflammation, and T cell immune exhaustion, and alters HIV infection in part through engagement with the T cell immunoglobulin mucin domain-3 (Tim-3) receptor and protein disulfide isomerases (PDI). Gal-9 was
The CD4 antigen: physiological ligand and HIV receptor.
Q J Sattentau et al.
Cell, 52(5), 631-633 (1988-03-11)

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