추천 제품
![(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide ≥98%](/deepweb/assets/sigmaaldrich/product/structures/404/859/0c6eb80d-2c3c-4bf1-98e8-1b319b597b12/640/0c6eb80d-2c3c-4bf1-98e8-1b319b597b12.png)
생물학적 소스
rabbit
결합
unconjugated
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
양식
buffered aqueous solution
분자량
antigen ~70 kDa
종 반응성
human, rat (predicted), mouse
농도
~1.0 mg/mL
기술
indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 cells
western blot: 0.5-1 μg/mL using whole extracts of HEK-293T cells expressing recombinant human EDEM2
UniProt 수납 번호
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... EDEM2(55741)
mouse ... Edem2(108687)
rat ... Edem2(296304)
일반 설명
ER degradation-enhancing α-mannosidase-like 2 is an enzyme encoded by the EDEM2 gene in humans. EDEM2 is localized to the endoplasmic reticulum (ER) mainly as a soluble glycoprotein. It is an enzyme encoded by the EDEM2 gene in humans. It is one of the ER-stress-induced members of the glycosyl hydrolase 47 family.
면역원
synthetic peptide corresponding to amino acids 22-38 of human EDEM2, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and 2 amino acids in rat EDEM2.
애플리케이션
Anti-EDEM2 (N-terminal) antibody produced in rabbit is suitable for indirect immunofluorescence at a concentration of 5-10μg/mL using mouse 3T3 cells and western blotting at a concentration of 0.5-1μg/mL using whole extracts of HEK-293T cells expressing recombinant human EDEM2.
생화학적/생리학적 작용
Overexpression of ER degradation-enhancing alphamannosidase-like protein 2 (EDEM2) accelerates ER associated degradation (ERAD) by promoting the release of terminally misfolded glycoproteins from the calnexin cycle, without affecting the rate of degradation of nonglycosylated polypeptides or the maturation of model secretory proteins.
물리적 형태
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
가장 최신 버전 중 하나를 선택하세요:
Role of EDEM in the release of misfolded glycoproteins from the calnexin cycle
Molinari M, et al.
Science, 299(5611), 1397-1400 (2003)
Steven W Mast et al.
Glycobiology, 15(4), 421-436 (2004-11-13)
In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome in a process known as ER-associated degradation (ERAD). Early in this pathway, a proposed lumenal ER lectin, EDEM, recognizes misfolded glycoproteins in the
Silvia Olivari et al.
FEBS letters, 581(19), 3658-3664 (2007-05-15)
Proteins synthesized in the endoplasmic reticulum (ER) lumen are exposed to several dedicated chaperones and folding factors that ensure efficient maturation. Nevertheless, protein folding remains error-prone and mutations in the polypeptide sequence may significantly reduce folding-efficiency. Folding-incompetent proteins carrying N-glycans
Silvia Olivari et al.
The Journal of biological chemistry, 280(4), 2424-2428 (2004-12-08)
Proteins expressed in the endoplasmic reticulum (ER) are subjected to a tight quality control. Persistent association with ER-resident molecular chaperones prevents exit of misfolded or incompletely assembled polypeptides from the ER and forward transport along the secretory line. ER-associated degradation
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