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E7783

Sigma-Aldrich

p3XFLAG-CMV™-13 Expression Vector

shuttle vector for transient or stable extracellular expression of C-terminal 3xFLAG

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모든 사진(2)

About This Item

UNSPSC 코드:
12352200

태그

3X FLAG tagged

Grade

for molecular biology

형태

buffered aqueous solution

펩타이드 태그 위치

C-terminal

배송 상태

dry ice

저장 온도

−20°C

일반 설명

The p3XFLAG-CMV™-13 Expression Vector is a 6.3 kb derivative of pCMV5 used to establish expression of transient or unstable C-terminal 3XFLAG™fusion proteins in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) downstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG?M2 antibody. Efficiency of replication and genomic integration is optimal when using an SV40 T antigen-expressing host.

p3XFLAG-CMV-7-BAP Control Plasmid is a 6.2 kb derivative of pCMV51 used for transient intracellular expression of N-terminal 3X-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp- Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein.

Vector Maps and Sequences

성분

  • p3XFLAG-CMV™-13 Expression Vector 20 μg (E4776) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
  • p3XFLAG-CMV™-7-BAP Control Plasmid 20 μg (C7472) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

원리

The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG®-fusion constructs. The preprotrypsin leader sequence precedes the FLAG® sequence.

법적 정보

This product is covered by the following patents owned by Sigma-Aldrich Co. LLC: US6,379,903, US7,094,548, JP4405125,EP1220933, CA2386471 and AU774216.
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
p3xFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC

관련 제품

제품 번호
설명
가격

Storage Class Code

10 - Combustible liquids

시험 성적서(COA)

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