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Merck
모든 사진(1)

주요 문서

E7407

Sigma-Aldrich

ERK1, active, untagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

동의어(들):

MAPK3, P44ERK1, P44MAPK, PRKM3

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About This Item

MDL number:
UNSPSC 코드:
12352200
NACRES:
NA.77

재조합

expressed in baculovirus infected Sf9 cells

Quality Level

제품 라인

PRECISIO® Kinase

분석

≥70% (SDS-PAGE)

양식

buffered aqueous glycerol solution

특이 활성도

329-445 nmol/min·mg

분자량

~44 kDa

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−70°C

유전자 정보

human ... MAPK3(5595)

일반 설명

Extracellular signal-regulated kinase 1 (ERK1) belongs to mitogen-activated protein kinases (MAPK)/ERK family of intermediate proteins in the signaling pathways. This gene contains nine exons and eight introns, and spans 9kb. It is a constitutively expressed protein, the activity of which is altered by the phosphorylation state. It has a higher expression level in nervous tissues. It contains 11 kinase subdomains, common between humans and mice. It has a molecular weight of 44kDa.

생화학적/생리학적 작용

ERK1 (MAPK3) participates in cellular signaling cascades that are activated in response to numerous growth factors and cytokines. Disruption of MAP kinase signaling functionality is associated with disease states including inflammation, cancer and neurodegenerative disease.
Extracellular signal-regulated kinase 1 (ERK1) act as intermediate in multiple signaling cascades, and is acted upon by MEK protein. MEK are essential for Golgi fragmentation during mitosis, and this is achieved by its action upon an alternatively spliced isoform of ERK1, called ERK1c. ERK1/2 signaling plays a key role in cellular response to topoisomerase II poisoning, by activating G2/M checkpoint, and thus, prevents poison-induced cell death. In melanocytes, pleiotrophin suppresses pigmentation or melanogenesis, by degradation of MITF, through the activation of ERK1/2 signaling.

물리적 형태

Supplied in 25 mM MOPS, pH 7.5, with 300 mM NaCl, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol

법적 정보

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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문서 라이브러리 방문

Yoav D Shaul et al.
The Journal of cell biology, 172(6), 885-897 (2006-03-15)
Extracellular signal-regulated kinase 1c (ERK1c) is an alternatively spliced form of ERK1 that is regulated differently than other ERK isoforms. We studied the Golgi functions of ERK1c and found that it plays a role in MEK-induced mitotic Golgi fragmentation. Thus
Daniel M Aebersold et al.
Molecular and cellular biology, 24(22), 10000-10015 (2004-10-29)
Extracellular signal-regulated kinases (ERKs) are signaling molecules that regulate many cellular processes. We have previously identified an alternatively spliced 46-kDa form of ERK1 that is expressed in rats and mice and named ERK1b. Here we report that the same splicing
F García et al.
Genomics, 50(1), 69-78 (1998-06-20)
The complete genomic structure of the human p44(mapk) gene (HMGW-approved symbol PRKM3) has been determined. The gene covers 9 kb and is composed of nine exons and eight introns. This structure is identical to the previously reported mouse p44(mapk) gene
Ryan H Kolb et al.
PloS one, 7(11), e50281-e50281 (2012-11-21)
Topo II poisons, which target topoisomerase II (topo II) to generate enzyme mediated DNA damage, have been commonly used for anti-cancer treatment. While clinical evidence demonstrate a capability of topo II poisons in inducing apoptosis in cancer cells, accumulating evidence
T G Boulton et al.
Biochemistry, 30(1), 278-286 (1991-01-08)
In rat 1 fibroblasts, insulin has little or no stimulatory effect on the activities of either MAP2 protein kinase or ribosomal protein S6 kinase. In contrast, in rat 1 cells that overexpress the normal human insulin receptor (rat 1 HIRc

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