콘텐츠로 건너뛰기
Merck
모든 사진(2)

주요 문서

모든 문서 보기

E7158

Sigma-Aldrich

pFLAG-CMV-4 Expression Vector

shuttle vector for intracellular transient or stable expression of N-terminal Met-FLAG

로그인조직 및 계약 가격 보기
모든 사진(2)

About This Item

UNSPSC 코드:
12352200

태그

Met-FLAG tagged

Grade

for molecular biology

형태

buffered aqueous solution

펩타이드 태그 위치

N-terminal

배송 상태

dry ice

저장 온도

−20°C

일반 설명

pFLAG-CMV™-4 is a 6.2 kb expression vector used for transient or stable expression in mammalian cells. The vector is a derivative of pCMV5 used for transient or stable expression of a properly inserted open reading frame as an N-terminal Met-FLAG® fusion protein.

The pFLAG-CMV-4 Expression Vector is a shuttle vector containing both bacterial and SV40 origins of replication for propagation in both E.coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using host cells that express the SV40 large T antigen.

The FLAG epitope is a small, hydrophilic 8 amino acid tag (DYKDDDDK) that provides for sensitive detection and high quality purification using ANTI-FLAG products available for Sigma Aldrich.

pFLAG-CMV-4-BAP Control Plasmid is a 7.7 kb derivative of pCMV5 used for transient intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.

Vector Maps and Sequences

성분

  • pFLAG-CMV™-4 Expression Vector 20 μg (E1775) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
  • pFLAG-CMV™-4-BAP Control Plasmid 20 μg (C4722) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

원리

The promoter-regulatory region of the human cytomegalovirus2 drives transcription of FLAG®-fusion constructs. The aminoglycoside phosphotransferase II gene (neo-r) confers resistance to aminoglycosides such as G 418 allowing for selection of stable transfectants.

법적 정보

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC

관련 제품

제품 번호
설명
가격

Storage Class Code

10 - Combustible liquids

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Chunxue Lu et al.
PloS one, 8(7), e68764-e68764 (2013-07-31)
Glycogen has been localized both inside and outside Chlamydia trachomatis organisms. We now report that C. trachomatis glycogen synthase (GlgA) was detected in both chlamydial organism-associated and -free forms. The organism-free GlgA molecules were localized both in the lumen of
M G Chaitra et al.
Journal of medical microbiology, 56(Pt 4), 466-474 (2007-03-22)
The PE and PPE proteins of Mycobacterium tuberculosis form a source of antigenic variation among different strains of M. tuberculosis. One of the PE_PGRS proteins, Rv1818c, plays a role in the pathogenesis of mycobacterial infection and specifically influences host-cell responses
Toru Furukawa et al.
Molecular cancer, 11, 88-88 (2012-12-12)
Pancreatic cancer is characterized by constitutive activation of mitogen-activated protein kinase (MAPK). Activation of MAPK is associated with the upregulation of genes implicated in the proliferation and survival of pancreatic cancer cells. We hypothesized that knockdown of these MAPK-associated molecules
Florence Baychelier et al.
Blood, 122(17), 2935-2942 (2013-08-21)
With an array of activating and inhibitory receptors, natural killer (NK) cells are involved in the eradication of infected, transformed, and tumor cells. NKp44 is a member of the natural cytotoxicity receptor family, which is exclusively expressed on activated NK
PML-RARα enhances constitutive autophagic activity through inhibiting the Akt/mTOR pathway.
Huang, Y., et al.
Autophagy, 7, 1-1 (2011)
모든 관련된 서류 보기

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.