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Merck
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Key Documents

E5529

Millipore

EZview Red Streptavidin Affinity Gel

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About This Item

UNSPSC 코드:
41106500
NACRES:
NA.56

형태

suspension

Quality Level

유통기한

1 yr

기술

western blot: suitable

기질

4% agarose

pH

7.2

용량

~10 μg(biotin per ml of packed gel)

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

The EZ View Red Streptavidin Affinity gel is composed of streptavidin, covalently attached to
cyanogen bromide-activated 4% agarose beads. It is designed to capture (pull-down) the biotinylated target molecules, such as proteins, peptides, antibodies, nucleic acids, lectins, receptors and ligands.

애플리케이션

Sutitable for use with immunoprecipitation, western blotting,and enzyme assays.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

특징 및 장점

  • Increased visibility - Red color reduces risk of incidental aspiration
  • Improved recovery of target protein by reduced accidental loss
  • Higher reproducibility - More consistent yields

물리적 형태

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

법적 정보

EZview is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

Alicia M Bostwick et al.
Biochemical and biophysical research communications, 528(3), 580-585 (2020-06-09)
Mammalian cells contain genetic information in two compartments, the nucleus and the mitochondria. Mitochondrial gene expression must be coordinated with nuclear gene expression to respond to cellular energetic needs. To gain insight into the coordination between the nucleus and mitochondria
Marion Mussbacher et al.
Scientific reports, 9(1), 15403-15403 (2019-10-30)
Murine 3T3-L1 adipocytes share many similarities with primary fat cells and represent a reliable in vitro model of adipogenesis. The aim of this study was to probe the effect of S-nitrosoglutathione (GSNO) on adipocyte differentiation. Adipogenesis was induced with a
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Cells, 11(1) (2022-01-12)
Pex11, an abundant peroxisomal membrane protein (PMP), is required for division of peroxisomes and is robustly imported to peroxisomal membranes. We present a comprehensive analysis of how the Pichia pastoris Pex11 is recognized and chaperoned by Pex19, targeted to peroxisome
Ting-Yu Chang et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 138, 111485-111485 (2021-03-20)
Aberrant alteration of epigenetic information disturbs chromatin structure and gene function, thereby facilitating cancer development. Several drugs targeting histone deacetylases (HDACs), a group of epigenetic enzymes, have been approved for treating hematologic malignancies in the clinic. However, patients who suffer
Lu Su et al.
The EMBO journal, 38(8) (2019-03-13)
SREBPs are master regulators of lipid homeostasis and undergo sterol-regulated export from ER to Golgi apparatus for processing and activation via COPII-coated vesicles. While COPII recognizes SREBP through its escort protein SCAP, factor(s) specifically promoting SREBP/SCAP loading to the COPII

관련 콘텐츠

Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.