E2906

Anti-dEGF Receptor, Extracellular Domain antibody, Mouse monoclonal

clone C-273, purified from hybridoma cell culture

• 동의어(들): Anti-Drosophila Epidermal Growth Factor Receptor, Anti-dEGFR
• MDL number: MFCD08705583
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: C-273, monoclonal
• 형태: buffered aqueous solution
• 종 반응성: Drosophila
• 농도: ~1.2 mg/mL
• 기술: immunohistochemistry: suitable; indirect ELISA: suitable; western blot: 0.25-0.5 μg/mL using recombinant protein representing amino acids 299-359 of the Drosophila EGFR extracellular domain
• 동형: IgG1
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: Drosophila melanogaster ... Egfr(37455)

설명

일반 설명

Anti-dEGF Receptor, Extracellular Domain antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma C-273 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a recombinant protein encoding at the extracellular portion of Drosophila epidermal growth factor receptor (EGFR). Drosophila EGF receptor, also known as Torpedo or DER has four ligands: Gurken, Spitz (the principle ligand), Vein and Argos. Two accessory proteins modulate its signalling: Rhomboid and Star.

애플리케이션

Suitable for the following:

• Immunohistochemistry
• Indirect ELISA
• Western blotting at a concentration of 0.25-0.5μg/mL using recombinant protein representing amino acids 299-359 of the Drosophila EGFR extracellular domain

생화학적/생리학적 작용

Drosophila epidermal growth factor receptor (EGFR) is involved in many developmental processes such as: egg polarity, cell identity in the ventral ectoderm, neurogenesis, development of the Malpighian tubules, and larval eye and wing development. The downstream signalling molecules that are activated by the Drosophila EGF receptor include: Src homology (Shc), DRK (a homolog of mammalian Grb2), a guanine nucleotide exchange factor (SOS) activated by DRK, and downstream targets including orthologs of Ras, Raf, and Rolled (MAP Kinase).

The epidermal growth factor receptor (EGFR) binds to its cognate ligands that lead to autophosphorylation of receptor tyrosine kinase and subsequent activation of signal transduction pathways that are involved in regulating cellular proliferation, differentiation and survival. EGFR is overexpressed in tumor cell lines and is associated with poor prognosis leading to decreased survival. Its activation also plays a role in resistance to chemotherapy and radiation treatment in tumor cells. EGFR tyrosine kinase inhibitors (TKIs) may act as a new treatment option for advanced lung adenocarcinoma. Its ligands function in the Drosophila CNS and maintains glial survival. Drosophila EGF receptor plays a critical role during the development of Drosophila retina.

물리적 형태

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable