추천 제품
생물학적 소스
rabbit
결합
unconjugated
항체 형태
IgG fraction of antiserum
항체 생산 유형
primary antibodies
클론
polyclonal
형태
buffered aqueous solution
분자량
antigen ~35 kDa
종 반응성
human
기술
immunohistochemistry: 1:250 using human cerebral cortex sections
western blot: 1:250-1:500 using PC-12 cell lysate
UniProt 수납 번호
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... EIF2S1(1965)
일반 설명
The eukaryotic translation initiation factor 2 subunit α (EIF2A) gene is mapped to human chromosome 14q23.3. The eukaryotic translation initiation factor 2 (eIF2), a multimeric protein is composed of three subunits termed α, β, and γ.
특이성
Anti-eIF2α (also known as eIF-2A) recognizes human eIF2α.
애플리케이션
Anti-eIF2α antibody produced in rabbit has been used in western blotting.
Anti-elF2α antibody is also suitable for immunohistochemistry at a working dilution of 1:250 using human cerebral cortex sections.
Anti-elF2α antibody is also suitable for immunohistochemistry at a working dilution of 1:250 using human cerebral cortex sections.
Anti-eIF2α antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:250-1:500 using PC-12 cell lysate. It was used as a primary antibody to detect eIF2α in a study.
생화학적/생리학적 작용
Increased eIF2α phosphorylation has been observed in Alzheimer′s disease. Viral infections are also known to induce eIF2α phosphorylation.
The main role of eIF2 complex is to transfer Met-tRNAi in the presence of GTP to the 40S ribosomal subunit during the initiation of translation. Regulation of the initiation of translation at the level of eIF2 involves the phosphorylation of eIF2α at a conserved Ser51 residue. This leads to inhibition of eIF2β activity, thus reducing guanine nucleotide exchange and consequently, the translation rate. Protein synthesis inhibition at the level of eIF2α phosphorylation is linked to a variety of cell stress signaling pathways via specific kinases. Phosphorylation of eIF2α reduces the rate of translation and gives the cell time to correct the stress damage, and selectively enhance the translation of genes important for stress remedy.
물리적 형태
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
저장 및 안정성
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
R C Wek et al.
Biochemical Society transactions, 34(Pt 1), 7-11 (2005-10-26)
In response to environmental stresses, a family of protein kinases phosphorylate eIF2 (eukaryotic initiation factor 2) to alleviate cellular injury or alternatively induce apoptosis. Phosphorylation of eIF2 reduces global translation, allowing cells to conserve resources and to initiate a reconfiguration
R C Wek
Trends in biochemical sciences, 19(11), 491-496 (1994-11-01)
Phosphorylation of eukaryotic initiation factor-2 (eIF-2) is an important mechanism regulating general translation initiation. Two mammalian eIF-2 kinases, the double-stranded-RNA-dependent kinase (PKR) and heme-regulated inhibitor kinase (HRI), have been characterized by sequencing, revealing shared sequence and structural features distinct from
Christopher G Proud
Seminars in cell & developmental biology, 16(1), 3-12 (2005-01-22)
Eukaryotic initiation factor eIF2 and its 'exchange factor' eIF2B play a key role in the regulation of protein synthesis in eukaryotes from yeast to mammals. Phosphorylation of eIF2 inhibits eIF2B and thus translation initiation. Four eIF2 kinases are now known
Rita Spilka et al.
Cancer letters, 340(1), 9-21 (2013-07-09)
Eukaryotic gene expression is a complicated process primarily regulated at the levels of gene transcription and mRNA translation. The latter involves four main steps: initiation, elongation, termination and recycling. Translation regulation is primarily achieved during initiation which is orchestrated by
Anton A Komar et al.
International journal of molecular sciences, 21(6) (2020-03-21)
Initiation of protein synthesis in eukaryotes is a complex process requiring more than 12 different initiation factors, comprising over 30 polypeptide chains. The functions of many of these factors have been established in great detail; however, the precise role of
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