D219

Monoclonal Anti-Dihydropyridine Receptor (α₂ Subunit) antibody produced in mouse

clone 20A, ascites fluid, buffered aqueous solution

• 동의어(들): Anti-CACNA2, Anti-CACNL2A, Anti-CCHL2A, Anti-LINC01112, Anti-lncRNA-N3
• MDL number: MFCD01323189
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: ascites fluid
• 항체 생산 유형: primary antibodies
• 클론: 20A, monoclonal
• 형태: buffered aqueous solution
• 분자량: antigen 143 kDa (reduced); antigen 220 kDa (non-reduced)
• 종 반응성: rat, mouse, rabbit, guinea pig
• 기술: immunohistochemistry (frozen sections): 1:500; western blot: 1:500
• 동형: IgG2a
• UniProt 수납 번호: O08532
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Cacna2d1(12293) ; rat ... Cacna2d1(25399)

설명

일반 설명

Dihydropyridine Receptor (DHPR) is a heteropentamer calcium ion channel and comprises of α1,α2/δ,β and γ subunits. It is localized in the skeletal muscle in the t-tubule membrane. The α2subunit of DHPR is a glycosylated subunit and is anchored on the membrane.

특이성

Reacts specifically with the α₂ subunit of the DHP receptor. Strong reactivity is observed with the α₂ subunit in rat, mouse, guinea pig, rabbit skeletal and cardiac muscle, and rat brain and lung tissue. Weaker reactivity is observed with rat kidney, liver and spleen tissues. By immunohistochemistry, double rows of discrete punctate staining represent pairs of triads on the opposing sides of the Z-lines.

면역원

Rabbit dihydropyridine (DHP) receptors, (α2 subunit)

애플리케이션

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal Anti-Dihydropyridine Receptor (α2 Subunit) antibody produced in mouse has been used in:

• immunofluorescent histochemistry
• surface plasmon resonance binding
• immunohistochemistry (IHC)
• immunoblotting

Use of this antibody for immunoblot analysis will allow for detection of a 220 kDa band (native conditions) or a 143 kDa band (denaturing conditions).Use of this antibody for immunohistochemistry of muscle tissue will demonstrate double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines are observed.

생화학적/생리학적 작용

Dihydropyridine Receptor (DHFR) α1 subunit is the voltage-sensor. DHPR interacts with ryanodine receptor (RyR) and also mediates excitation-contraction (EC) coupling in cardiac and skeletal muscles. Mutation in the DHPR (E1014K) affects its affinity and selectivity towards calcium permeation. Additionally, it also plays a key role in the synaptic transmission.

물리적 형태

Solution in phosphate buffered saline containing 0.05% sodium azide.

기타 정보

This mouse monoclonal IgG2a antibody is supplied as a mouse ascites diluted in phosphate buffered saline containing 0.05% sodium azide as a preservative.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)