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Merck
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CS0760

Sigma-Aldrich

Isolated Mitochondria Staining Kit

1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

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About This Item

EC Number:
217-896-6
UNSPSC 코드:
12352207
NACRES:
NA.32

Quality Level

200

사용

 kit sufficient for 1000 reactions (using 96 multiwell plates)
 kit sufficient for 50 reactions (in a 2 mL cuvette)

포장

pkg of 1 kit

저장 조건

dry at room temperature

기술

flow cytometry: suitable
protein staining: suitable

형광

λex 490 nm; λem 590 nm (JC-1 dye)

응용 분야

cell analysis
detection

검출 방법

fluorometric

배송 상태

dry ice

저장 온도

−20°C

관련 카테고리

애플리케이션

Isolated Mitochondria Staining Kit has been used to assess mitochondrial inner membrane potential.

특징 및 장점

  • Fast, simple, and convenient method for staining isolated mitochondria and for assaying mitochondria intactness
  • Contains all the reagents required for the detection of changes in mitochondrial inner-membrane electrochemical potential
  • Contains the antibiotic Valinomycin that permeabilizes the mitochondrial membrane, and thus, dissipates the mitochondrial electrochemical potential. Valinomycin can be used as a control agent that prevents JC-1 aggregation

원리

The kit is based on mitochondria staining using the JC-1 dye. In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) does not allow the accumulation of JC-1 dye in the mitochondria. The dye is detected using a fluorimeter or fluorescence microscope.

분석 메모

The kit measures mitochondria intactness and detects events that dissipate the mitochondrial membrane potential.

키트 구성품 전용

제품 번호
설명
  • DMSO 1 mL
  • JC-1 Assay Buffer 5X 25 mL
  • JC-1 Stain 25 μg
  • Valinomycin Ready Made 100 μL

픽토그램

Skull and crossbones
GHS06

신호어

Danger

유해 및 위험 성명서

H300 + H310

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 1 Oral

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
108M4080V
11180552
038M4176V
11171115
116M4090V

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

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문서

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Mitochondrial Stress and ROS

Oxidative stress is mediated, in part, by reactive oxygen species produced by multiple cellular processes and controlled by cellular antioxidant mechanisms such as enzymatic scavengers or antioxidant modulators. Free radicals, such as reactive oxygen species, cause cellular damage via cellular.

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