추천 제품
생물학적 소스
human female ovary (Source Disease: Ovarian adenocarcinoma)
Quality Level
OMIM 수납 번호
저장 온도
−196°C
유전자 정보
human ... STAT3(6774)
일반 설명
SKOV3 GFP-STAT3 are ovarian adenocarcinoma cells from a human 64 year old caucasion female having a ZFN modification creating a GFP-STAT3 transgene expressed from the endogenous STAT3 gene locus.
This cell line was derived from ATCC Catalog No. HTB-77.
This cell line was derived from ATCC Catalog No. HTB-77.
애플리케이션
This product is a human SKOV3 cell line in which the genomic STAT3 gene has been endogenously tagged with a Green Fluorescent Protein (GFP) gene using CompoZr® zinc finger nuclease technology. Integration resulted in endogenous expression of the fusion protein in which GFP is attached to the N-terminus of STAT3. Fluorescence imaging shows GFP-STAT3 expression throughout the cell. Upon activation with the ligand IL-6, the cell line shows redistribution of STAT3 from the cytoplasm to the nucleus, making it useful for high content screening of compounds that modulate STAT3 activity. This stable cell line was expanded from a single clone. The target′s gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.
To learn more, please visit the Cellular Reporter Cell Line webpage
To learn more, please visit the Cellular Reporter Cell Line webpage
특징 및 장점
Zinc Finger Nuclease (ZFN)-mediated targeted integration of a fluorescent GFP tag just behind the start codon of the STAT3 gene on chromosome 17q21.2 to create a cell line exhibiting stable expression of the transgene tagged with GFP on the N-terminus of the protein.
These SKOV3 cells are adherent with a doubling time of approx. 48 hours.
These SKOV3 cells are adherent with a doubling time of approx. 48 hours.
품질
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.
제조 메모
Media Renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3-1:6
The base medium for this cell line is McCoy′s 5A Medium, Cat. No. M8403. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F2442, to a final concentration (v/v) of 10% and L-glutamine, Cat. No. G7513, at a final concentration of 1.5 mM.
Cell freezing medium-DMSO 1X, Cat. No. C6164.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3-1:6
The base medium for this cell line is McCoy′s 5A Medium, Cat. No. M8403. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F2442, to a final concentration (v/v) of 10% and L-glutamine, Cat. No. G7513, at a final concentration of 1.5 mM.
Cell freezing medium-DMSO 1X, Cat. No. C6164.
법적 정보
CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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