ATDC5

99072806, mouse unknown/unspecified, Epithelial-like

• 동의어(들): ATDC-5 Cells
• UNSPSC 코드: 41106514

속성

• product name: ATDC5, 99072806
• 생물학적 소스: mouse unknown/unspecified
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial-like
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse 129 teratocarcinoma AT805 derived

세포주 설명

The chondrogenic cell line ATDC5 has been derived from teratocarcinoma AT805. Cells showed a sequential transition of phenotype in vitro. They encompass stages from mesenchymal condensation to calcification. Bone morphogenetic protein-2 (BMP-2) stimulated the sequential progression of early- and late-phase differentiation. Activation of parathyroid hormone (PTH)/PTH-related peptide (PTHrP) receptor resulted in inhibition of this differentiation capacity.

애플리케이션

ATDC5 has been used to study cartilage differentiation.

배양 배지

DMEM: Ham′s F12 (1:1) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) i.e. seeding at 1-3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA;CO₂; 37°C subculture every 2-3 days.

기타 정보

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