GM1899A

98120701, human unknown/unspecified, Lymphoblastoid

• UNSPSC 코드: 41106514

속성

• product name: GM1899A, 98120701
• 생물학적 소스: human unknown/unspecified
• 성장 모드: Suspension
• 핵형: Not specified
• 형태학: Lymphoblastoid
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human lymphoblast, HPRT - deficient

세포주 설명

The lymphoblastoid cell line GM1899A has been derived from a Lesch-Nyhan patient. It is deficient for hypoxanthine-guanine phosphoribosyl transferase (HPRT) with a point mutation in the respective gene. This cell line is used as a feeder for extended-term culture of human T-lymphocytes (see LB lines in the collection).

애플리케이션

Feeder for T-lymphocyte culture

배양 배지

Dutch Modified RPMI 1640 + 2mM Glutamine + 20mM Hepes + 2mM Sodium Pyruvate (NaPy) + 20% Heated Inactivated Foetal Bovine Serum (HI-FBS)

계대배양 정규 작업

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C. Do not dilute below 1 x 100,000 cells/ml, wash 2-3 times after resuscitation before seeding; disaggregate every few days by pipetting to avoid formation of large aggregates

기타 정보

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