CTX TNA2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 98102213, rat brain, Neuronal (astrocyte)

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: rat brain
• 포장: tube of 5 μg 98102213-DNA-5UG; pkg of vial of cells 98102213-1VL
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Neuronal (astrocyte)
• 제품: Alpha 2 macroglobulin
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Rat Astrocyte, Transfected

세포주 설명

Established by transfecting cultures of primary astrocytes from brain frontal cortex tissue of 1 day old sprague-dawley rats with a DNA construct containing the oncogenic early region of SV40. Transcriptional control was effected using the human GFAP promoter (pGFA-SV-TE) and the murine phosphoglycerate kinase promoter (pPGK-neo). Cloning was achieved using G418. The cells are phenotypically similar to type 1 astrocytes. CTX TNA2 displays 20% positive staining for glial fibrillary acid protein (GFAP) and a β alanine inhibitable high affinity uptake mechanism for gamma amino butyric acid (GABA). α-2-macroglobulin production is similar to that found in primary astrocytes, but transferrin production is reduced. The cell line has been shown not to produce proenkephalin A, galactoceebroside or to express the 04 or A2B5 epitopes characteristic of type 2 astrocytes. Immunostaining has shown the SV40 T antigen to be present in over 95% of cells.

애플리케이션

Studies of the development and biochemistry of the central nervous system.

배양 배지

DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Subculture before flasks become confluent or cells will detach in sheets reducing the effectiveness of trypsin

기타 정보

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