LB199

98062321, human blood, Lymphoblastoid

• UNSPSC 코드: 41106514

속성

• 제품명: LB199, 98062321
• 생물학적 소스: human blood
• 포장: tube of 5 μg 98062321-DNA-5UG; pkg of vial of cells 98062321-1VL
• 성장 모드: Suspension
• 핵형: Not specified
• 형태학: Lymphoblastoid
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human lymphocyte, trichothiodystrophy, EBV-transformed

세포주 설명

LB199 was derived from mononuclear cells from a peripheral blood sample from a 16 year old male. The patient was diagnosed with trichothiodystrophy and was shown to have a severe deficiency in excision repair of UV-irradiation damage. The defect was able to complement all known Xeroderma pigmentosum complementation groups and was not intragenic. This cell line is therefore a representative of a new excision-repair complementation group. The cell line was established by subsequent EBV transformation. Fibroblasts from the same individual have been designated TTD1BR. EBV-transformed lymphoblastoid cells from the mother are available as LB198 (Sigma Catalogue number. 98062320).

애플리케이션

DNA repair deficiency studies

배양 배지

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

계대배양 정규 작업

Maintain cultures between 3-9 x 100,000 cells/ml; dilute every 1 -2 days to 3 x 100,000 cells/ml; 5% CO₂; 37°C. Cells form floating aggregates.

기타 정보

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