A375-C5 Cell Line human

97011320, human skin, Epithelial-like

• UNSPSC 코드: 41106514

속성

• 제품명: A375-C5 Cell Line human, 97011320
• 생물학적 소스: human skin
• 설명: Human malignant melanoma IL-1 insensitive
• 포장: tube of 5 μg 97011320-DNA-5UG; pkg of vial of cells 97011320-1VL
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial-like
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human malignant melanoma IL-1 insensitive

세포주 설명

The cell line A375-C5 is unresponsive to the anti-proliferative effect of IL-1. It has been derived from the parental line A373 (Sigma catalogue no. 88113005) which was subjected to cloning by limiting dilution. This method resulted in the establishment of A375-C5 and an IL-1 sensitive clone, A375-C6 (Sigma catalogue no. 97011321). The unresponsiveness of A375-C5 is probably due to an IL-1 receptor deficiency. The cells have been eradicated from mycoplasma at ECACC.

애플리케이션

IL-1 resistance studies, receptor studies

배양 배지

RPMI 1640 + 2mM Glutamine + 10% FBS

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:5 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

기타 정보

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