b.End5

96091930, mouse brain, Endothelial-like

• 동의어(들): Brain-derived Endothelial 5 Cells, bEnd3 Cells, bEnd5 Cells
• UNSPSC 코드: 41106514

속성

• 제품명: b.End5, 96091930
• 생물학적 소스: mouse brain
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Endothelial-like
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse Balb/c brain endothelioma

세포주 설명

The cell line b.End5 has been established from brain endothelial cells of BALB/c mice. Immortalisation has been carried out by infection of primary cells with retrovirus coding for the Polyoma virus middle T-antigen. b.End5 cells are positive for endothelial specific proteins (PECAM-1, Endoglin, MECA-32, Flk-1) tested by FACS. Inflammatory cytokines induce the expression of proteins such as CAM-1, VCAM-1 and E-selectin. Injection into mice causes development of haemangiomas.

애플리케이션

Study of endothelial cell growth, differentiation and influence of oncogenes.

배양 배지

DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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