OE33 Cell Line human

Caucasian esophageal carcinoma, 96070808

• 동의어(들): JROECL33, JROECL33 Cells, OE-33 Cells
• UNSPSC 코드: 41106514

속성

• 생물학적 소스: human esophagus
• 성장 모드: Adherent
• 핵형: Aneuploid
• 형태학: Epithelial
• 제품: Not specified
• 수용체: MHC class I, ICAM-1
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian oesophageal carcinoma

세포주 설명

The cell line OE33, also known as JROECL33, was established from the adenocarcinoma of the lower oesophagus (Barrett′s metaplasia) of a 73 year old female patient. The tumour was identified as pathological stage IIA (UICC) and showed poor differentiation. OE33 express HLA-A, -B and -C antigens (MHC class I) and ICAM-1 constitutively. Expression of HLA-DR (MHC class II) can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice.

애플리케이션

OE33 cell line has been used to study the effect of microRNA-MiR-193b on autophagy and cell death on these cells. It has also been used to study the sensitivity of the cells to anti-c-Met monoclonal antibody ABT-700.

Study of oesophageal cancer

배양 배지

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:8 i.e. seeding at 1x10,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C. Initially these cells grow slowly and can take up to 7 days until ready for the next split, 50% media changes will be necessary every 2-3 days (i.e. replacing half the old medium with fresh).

기타 정보

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