NCI-H69/LX4

96042329, human lung, Not specified

• UNSPSC 코드: 41106514

속성

• product name: NCI-H69/LX4, 96042329
• 생물학적 소스: human lung
• 성장 모드: Aggregates in suspension
• 핵형: Not specified
• 형태학: Not specified
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian small cell lung carcinoma, drug-resistant

세포주 설명

NCI-H69/LX4 has been established by exposure of the parent line, H69 (Sigma Catalogue number. 91091802) to doxorubicin, also known as adriamycin. The line hyperexpresses P-glycoprotein and demonstrates a multidrug resistant drug accumulation deficit. It is recommended to culture the cells without drug after resuscitation until the first passage.

애플리케이션

Analysis of acquired drug-resistance, study of small cell lung cancer

DNA 프로파일

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 11,13
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17

배양 배지

RPMI + 2mM Glutamine + 0.4μg/ml doxorubicine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) by repeated pipetting of aggregates or scraping; 5% CO₂; 37°C

기타 정보

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.