EMT6/MTXR

96042322, mouse unknown/unspecified, Epithelial-like

• UNSPSC 코드: 41106514

속성

• product name: EMT6/MTXR, 96042322
• 생물학적 소스: mouse unknown/unspecified
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial-like
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse mammary, drug-resistant

세포주 설명

The drug-resistant cell line EMT6/MTXR was derived from the mouse mammary tumour cell line EMT6/P (Sigma Catalogue number. 96042344) by growth in increasing concentrations of methotrexate. EMT6/MTXR did not hyperexpress P-glycoprotein and did not show cross-resistance to other drugs of the multi-drug resistance (MDR) phenotype. It is recommended to culture the cells without drug on resuscitation until first passage or growth has been fully established.

애플리케이션

Drug-resistance studies

배양 배지

MEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1μg/ml methotrexate + 15% Foetal Bovine Serum (FBS).. Resuscitate in drug-free medium; add drug once growth is fully established

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA;5% CO₂; 37ºC

기타 정보

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