추천 제품
제품명
NCI-H510A, 96020944
생물학적 소스
human lung (adrenal metastasis)
성장 모드
Adherent/aggregates in suspension
핵형
Hypotriploid; modal number = 54; range = 46 to 57. Twenty to 25 marker chromosomes common to all cells. These include t(13q21q), der(1)t(1;21)(p36.1;q11), der(7)t(7;7)(p22;q22), 11p+, 12p+, and 15p+.
형태학
Not specified
제품
Not specified
수용체
Not specified
기술
cell culture | mammalian: suitable
관련 질환(들)
metastasis
배송 상태
dry ice
저장 온도
−196°C
세포주 기원
Human lung carcinoma, adrenal gland metastasis
세포주 설명
The NCI-H510A cell line was derived by D. Carney, A.F. Gazdar amd associates in 1982 from an adrenal metastasis in an adult, 56-year-old, male Caucasian patient. The cells produce easily detectable p53 mRNA at levels comparable to those in normal lung tissue. The line does not exhibit any gross structural DNA abnormalities. The cells express elevated levels of four biochemical markers of small cell lung carcinoma (SCLC) i.e. neuron specific enolase, the brain isoenzyme creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity. This cell line is tumourigenic in nude mice (the cells form transplantable tumours with typical small cell carcinoma histology).
배양 배지
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
계대배양 정규 작업
Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 2-4x10,000 cells/cm2; 5% CO2; 37°C. This cell line grows as a mixture of cells in suspension and adherent cells. Subcultures can be prepared by scraping the adherent cells into the medium,
기타 정보
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